发光细菌法在黄岛溢油污染海水遗传毒性评价中的应用.DOC

发光细菌法在黄岛溢油污染海水遗传毒性评价中的应用( 栾晓1 崔志松1(( 高伟1 文小龙2 徐广飞2 李倩1 孙承君1 郑立1 1国家海洋局第一海洋研究所海洋生态研究中心 青岛 266061 2青岛科技大学化学与分子工程学院 青岛 266042 摘 要 2013年11月22日，黄岛输油管线发生爆炸，胶州湾岸滩受到的溢油污染。Acinetobacter sp. RecA应用于溢油污染海水样品的毒性评价。研究发现，溢油1d后距离的S1、S2、S3站点海水样品的石油烃含量0.55 mg/L～1.04 mg/L其遗传毒性相当于0. mg/L～1.78 mg/LMMC）；海水中的石油烃含量随时间逐渐降低至＜0.02 mg/L，其遗传毒性也随之逐渐降低距离的S4、S5站点海水样品的石油烃含量一直较低（＜0.02 mg/L），表现为低毒或无毒。Genotoxicity evaluation of contaminated seawater in huangdao oil spill based on luminescent bacteria Acinetobacter sp. RecA * LUAN Xiao1, CUI Zhi-Song1**, GAO Wei1, Wen Xiao-long2, Xu Guang-fei2, Li Qian1, SUN Cheng-jun1, Zheng Li1 1Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061 2 Institute of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042 Abstract Objectives: Local beaches and sea water at Jiaozhou Bay was polluted by crude oil to different degrees after the Sinopec pipeline exploded on Nov. 22nd, 2013 at Huangdao, China. In order to investigate the ecological effect of petroleum hydrocarbons in seawater on marine organisms, a rapid genotoxicity detection method based on the luminescent bacteria Acinetobacter sp. RecA was developed in this study. Methods: The chemical composition of petroleum hydrocarbons in the seawater samples collected from five sites ranging from 1 km to 15 km away from the oil leakage point during a time course of 90 days were first analyzed by GC-MS. Meanwhile, the genotoxicity of each sample was determined by Acinetobacter sp. RecA using mitomycin C (MMC) as reference standard toxicant. Results: The petroleum hydrocarbon contents of 3 samples from sites S1, S2, and S3 within 1 km away from the oil leakage point were from 0.55 mg/L to 1.04 mg/L. The corresponding genotoxicities were equivalent to 0.60 mg/L to 1.78 mg/L of MMC. The genotoxicity level decreased as the petroleum hydrocarbon content gradually decreased to 0.02 mg/L over time. The samples from