催产素预处理对胎鼠海马脑片缺氧无糖损伤后OTR、GABAARβ1表达的影响.docVIP

　　催产素预处理对胎鼠海马脑片缺氧无糖损伤后OTR、GABAARβ1表达的影响 【摘要】 目的：研究催产素(OT)预处理对胎鼠海马脑片缺氧无糖损伤后催产素受体(OTR)、γ氨基丁酸A受体β1亚基(GABAARβ1)表达的影响，探讨OT神经保护的作用机制。方法： 采用胎龄20 d SD大鼠海马脑片，实验组经含10-6mol·L-1 OT人工脑脊液(ACSF)孵育预处理后行缺氧无糖损伤(OGD)，对照组经正常ACSF孵育后直接行OGD，各15 min，SP免疫组化法半定量测定海马CA1区OTR、GABAARβ1表达变化，电镜观察神经元损伤情况。结果： 实验组海马CA1区OTR、GABAARβ1表达均显著高于对照组(t=2.118、P=0.048;t=2.272、P=0.036)。对照组大量神经元受损,核固缩或碎裂，部分核膜溶解，实验组细胞核、粗面内质网、线粒体均较对照组有所改善,核膜较光滑,胞浆肿胀较对照组减轻。结论： OT上调胎鼠海马OTR、GABAARβ1表达，减少神经元变性坏死，增强大脑耐受缺氧损伤的能力，对胎儿中枢神经系统起保护作用。 【关键词】 催产素; 海马; 缺氧无糖损伤; 催产素受体; γ氨基丁酸A受体β1亚基 　　[Abstract] Objective： To study the effects of oxytocin(OT) on changes of oxytocin receptor(OTR), gammaamimobutyric acid receptor A β1 subunit(GABAAR β1) expressions in fetus rat hippocampus induced by oxygen and glucose deprivation(OGD), and to investigate the mechanism of neuroprotective effects of OT. Methods： Gestational age 20 days SD rat hippocampus slices in the experimental group underent incubation by containing 10-6 mol·L-1 OT artificial cerebrospinal fluid(ACSF), the control group al ACSF. Oxygen and glucose deprivation(OGD) in, respectively. SP immunohistochemistry and semiquantitative technique ine hippocampus CA1 area OTR, GABAAR β1 expressions, and observe neuronal damage in electron microscopy. Results: The expressions of OTR, GABAAR β1 in CA1 area of hippocampus ental qroup(t=2.118, P=0.048; t=2.272, P=0.036). A large number of damaged neurons , nuclear condensation or fragmentation, some of the nuclear membrane dissolved in the control group; ic reticulum, mitochondria proved, nuclear membrane ooth, cytoplasmic sental group. Conclusion: OT can increase hippocampal OTR, GABAAR β1 expressions, decrease neuronal degeneration and necrosis, enhance the capacity of the brain tolerance to hypoxia, and play the protective effects on fetal nervous system. 　　[Key pus; oxygen and glucose deprivation; oxytocin receptor; gammaamimobutyric acid receptor A β1 subunit 　　新生儿缺氧缺血性脑病(hypoxicischemic encephalopathy, HIE)是围产期缺氧、窒息导致脑的缺氧缺血性损害，临床出现一系