PROBDNF对大鼠后足切割疼痛的影响-中华医学会麻醉学分会.docVIP

PROBDNF对大鼠后足切割疼痛的影响 杨隆秋1李昌琪2常业恬1徐军美1戴茹萍1* 1中南大学湘雅二院麻醉科， 湖南 长沙 410011 中国 2中南大学人体解剖和神经生物学教研室， 湖南 长沙 410011 中国 *通讯作者 【摘要】　目的 研究大鼠后足切割后PROBDNF在脊髓和背根神经节的表达及注射PROBDNF后大鼠的行为学变化。方法 16只大鼠随机分为切割组和对照组，切割组建立后足切割模型，取两组大鼠的脊髓和背根神经节行免疫荧光实验;腹腔注射不同剂量的抗PROBDNF抗体，腹腔注射抗PROBDNF抗体和抗BDNF抗体，鞘内注射抗PROBDNF抗体和抗BDNF抗体，足底注射抗PROBDNF抗体，而后建立切割模型，行切割后机械刺激缩足阈值测定。结果 切割1小时后大鼠背根神经节PROBDNF的表达增强，脊髓PROBDNF的表达没有明显变化；腹腔注射抗PROBDNF10mg/kg对大鼠切割疼痛止痛作用最明显；鞘内注射抗BDNF有明显的止痛作用，腹腔注射抗PROBDNF有明显的止痛作用；足底注射抗PROBDNF止痛作用不明显。结论PROBDNF在转运至脊髓之前已经转变为成体BDNF或者与其受体结合，腹腔注射PROBDNF具有明显的止痛效果，并且其止痛作用有封顶效应。 【关键词】PROBDNF; 切割痛; 免疫荧光; 行为学 The effects of PROBDNF on cutting pain in rats with the hind paw incision Long-Qiu Yang1 ,Chang-Qi Li2, Chang-Ye Tian1,Jun-Mei Xu1 and Ru-Ping Dai1*Address: 1Department of Anesthesia, Xiang-Ya Second Hospital, Central South University, Changsha, PR China and 2Department of Anatomy and Neurobiology, Xiang-Ya College of Medicine, Central South University, Changsha, PR China Email:yanglongqiu@; Corresponding author Ru-Ping Dai* - Ruping_dai@ 【Abstract】　Objective To study PROBDNF’s expression in the spinal cord and dorsal root ganglia after rat’s hind paw incision and the changes of behavior after PROBDNF injection.Methods 16 rats were randomly divided into cutting group and control group. cutting model were set up in rats of cutting group . Immunofluorescence test detected the spinal cord and dorsal root ganglia from two groups of rats. We established cutting model and measured hind-paw withdrawal threshold after incision after intraperitoneal injection of different doses of anti-PROBDNF antibodies, intraperitoneal injection of anti-PROBDNF antibody and anti-BDNF antibody, intrathecal injection of anti-PROBDNF antibody and anti-BDNF antibody and plantar injection of anti-PROBDNF antibody.Results PROBDNF’s expression was enhanced in dorsal root ganglion 1 hour after incision and its expression in spinal cord had no significant change. Intraperitoneal injection of anti-PROBDNF10mg/kg had the most o