断藤益母汤含药血清对类风湿关节炎滑膜成纤维细胞增殖及TNF-α分泌的影响.PDF

17 2013 2 6 Rheumatism and Arthritis 2013 , Vol.2, No.6 1 2 2 梁　江 ，林昌松 ，林云斌 Ρ Ρ Ρ Ρ Effects of Serum Containing Duanteng Yimu Decoction on Proliferation of Fibroblast-like Synoviocytes and TNF-α Production in Patients with Rheumatoid Arthritis Liang Jiang,Lin Chang-song,Lin Yun-bin ABSTRACTObjective:To investigate the effects of serum containing duanteng yimu decoction (DYD) on proliferation of fi broblast-like synoviocytes (FLS) and TNF-α (tumor necrosis factor-α,TNF-α) production in rheumatoid arthritis patients.Methods:3 kinds of rabbits serum [normal,serum containing lefl unomide (LEF),serum containing DYD] were made.RA-FLS were separated from knee joints of RA patients and subcultured for 3-5 generations.RA-FLS cells were divided into 3 groups: the control group,the LEF group (serum containing LEF) and the DYD group (serum containing DYD) and then proliferation of cells in 3 groups were evaluated by MTT at three time points (24,48,72 h).After 72 h,cell culture medium was used to detect the TNF-α level with ELISA method.Results:Compared with the control group,after 48 h,72 h,Lefl unomide and DYD serums have obviously inhibitive effects o