温肾中药对原代培养正常人乳腺上皮细胞增殖的影响.PDF

中西医 结合 学报 2 0 06 年 7 月 第 4 卷 第 4 期 Jou r n al o f C hi ne se In t egr a tiv e M edicin e , Ju l y 20 0 6 , V ol .4 , N o .4 ·397 · 温 肾 中药 对 原 代 培 养 正 常 人 乳 腺 上 皮 细 胞 增 殖 的影 响 叶媚娜 , 陈红风 ( 上海 中医药大学龙华医院乳腺科 , 上海 200032 ) [摘要] 目的: 观察温肾中药对雌二醇和孕酮所致的原代培养正常人乳腺上皮细胞增殖的影响。方法 : 采用 消化法进行正常人乳腺上皮细胞分离及原代培养。在相差倒置显微镜和透射 电镜下对细胞进行形 态学鉴 定。应用雌二醇和孕酮干预以促使正常人乳腺上皮细胞增殖, 给予不同浓度温肾 中药及他莫昔芬进行药物 干预。采用 M T T 法检测不同浓度、不同药物干预下乳腺上皮细胞的增殖情况。结果 : 原代培养正常人乳腺 上皮细胞经形态学鉴定符合正常人乳腺上皮细胞的形态学特征。浓度均为 1 ×10 - 5 g/ L 的雌二醇和孕酮混 合培养液可以明显促进正常人乳腺上皮细胞增殖。高浓度温肾中药能明显促进原代培养正常人乳腺上皮细 胞增殖, 而低浓度温肾中药则可抑制其增殖。他莫昔芬对原代培养正常人乳腺上皮细胞增殖无 明显影响。 结论 : 雌二醇和孕酮可以促进正常人乳腺上皮细胞增殖。温肾中药调节正 常人乳腺上皮细胞增殖的作用 与 药物浓度相关。 [ 关键词] 温肾; 中草药; 原代培养; 乳腺, 人; 上皮细胞; 细胞增殖 [ 中图分类号] R 655 .82 [ 文献标识码] A [ 文章编号 ] 1672- 1977 ( 2006 ) 04-0397-0 5 Effects of Chinese herbal medicine for warming kidney on proliferat ion of normal human mammary epi thelial cells in pr imary cul ture Mei-Na YE, Hong-Feng CHEN (D epar tm en t o f Brea st Su r ger y , Lon ghua Hospital , Sh anghai Univer sity o f T r aditional Chine se Medicine , Shan gh ai 200032 , Chin a) ABSTRACT Obj ective: To evaluate the effects of Chinese herbal medic ine for warming kidney on prolifera- t ion of normal human mammary epithelial cells in primary culture . Methods : The normal human mammary epi- thelial cells were dissociated by digestion with collagenase type Ⅰ. The morphological identif ication of nor- mal human mammary epithelial cells in primary culture was determined under inverted phase contrast mic ro- scope and transmission elect ron microscope . The estradiol and progesterone were added to culture medium to induce the proliferation of normal human mammary epithelial cells in primary culture , and the different do- ses of Chinese herbal medicine for warming kidney and tamoxifen were also added into the culture medium . The MTT assay