Enhancement of L（＋）-Lactic Acid Production of Immobilized Rhizopus Oryzae Implanted by Ion Beams.pdfVIP

Plasma Science and Technology,Vo1．10，No．1，Feb．2008 Enhancement of L(+)一Lactic Acid Production of Immobilized Rhizopus Oryzae Implanted by Ion Beams FAN Yonghong(樊永红) ，一，YANG Yingge(杨英歌) ， ZHENG Zhiming(郑之明) LI Wen(李文) ，WANG Peng(_F鹏) ，YAO Liming(姚黎明) ， YU Zengliang(余增亮) Key Laboratory of Ion Beam Bioengineering，Institute of Plasma Physics Chinese Academy of Sciences，Hefei 230031 j China 。Xi~iang University，Urumqi 830046，China A bstract Immobilized Rhizopus oryzae culturing may be a solution to the inhibited production of L(+)一lactic acid in submerged fermentation，which is caused by aggregated mycelia floc．In the present study，a R．oryzae mutant(RL6041)with a 90％conversion rate of glucose into L—lactic acid was obtained by N+implantation under the optimized conditions of a beam energy of 15 keV and a dose of 2．6×10 ions／cm ．Using polyurethane foam as the immobilization matrix． the optimal L—lactic acid production conditions were determined as 4 mm polyurethane foam． 150 r／min，50 g／L一80 g／L of initial glucose，38。C and pH 6．0．15一cycle repeated productions of L—lactic acid by immobilized RL6041 were performed under the optimized culturing conditions and over 80％of the glucose was converted into L—lactic acid in 30 hours on average．The results show that immobilized RL6041 is a promising candidate for continuous L—lactic acid production． Keywords：immobilized Rhizopus oryzae，L—lactic acid，ion beam implantation PACS：41．85．Ar．61．72．W、v．61．82．Pv 1 Introduction turing．which can be easily re—used for lactic acid pro—