海洋真菌HZ-01菌株产几丁质酶的研究.pdfVIP

安徽农业科学，Jmmml 0fAnhui Agi．Sci．2008，36(4)：1330—1331，1334 责任编辑 孙红忠 责任校对 李晓玉 海洋真菌HZ．01菌株产几丁质酶的研究 王伟霞，李福后 (江苏省海洋生物技术重点建设实验室，淮海工学院海洋学院，江苏连云港222oo5) 摘要 [目的]探讨海洋真茵HZ-01菌株产几丁质酶的培养条件和部分性质。[方法]从海洋环境中筛选出1株几丁质酶高产真茵I-V_,- 01．以该菌株为材料，进行不同碳源、氮源以及温度对菌株发酵产生几丁质酶的影响试验，研究几丁质酶的培养条件。将粗酶液在不同 温度下保温6o min后，取样测定几丁质酶活力，分析几丁质酶的热稳定性和酸碱稳定性。[结果]海洋真茵HZ-01茵株产几丁质酶的最 佳碳源为几丁质，蛋白胨为最佳氮源；以1．O％的几丁质作为碳源，0．1％的蛋白胨作为氮源，温度为30℃时，其产生的几丁质酶活力最 高，且随着温度的上升，该酶活力下降；该酶的酸碱稳定性较差，只在pH值6～8具有较高活性。[结论]该研究为规模化生产几丁质酶 初步奠定基础。 关键词 海洋真茵；几丁质酶；发酵条件 中图分类号 Q936 文献标识码 A 文章编号 0517—661l(2OO8)04—01330—02 Studies on PI h n2 Oaitlmse withMa I忆加l SWain WANG Wei-xiaH al (Jiangsu Provincial Key tab of Marine Biotechnology，Ocean College，Huaihai Institute of Techrtology，Lianyungang，Jian~ 2220晒) Abstract[Objective] n purpose was to discuss the culture condition and characteristics for producing chitinase with marine fIlr】gi 加1 stl~Jn． [Method]A stl~．in ofh】gi托 1 with high yield of chitinasewasscreened outfrommarine environment，withwhichasmaterial，吐le experiment on吐 iIl— ttuenee of different eadxm(C)s0|Jroe，nitrogen(N)source and temperature on producing chitinase with the strain through fermentation was done to stuay the culture condition of chitinase．Afterthe crude enzynle solutionwastreatedwith heat preservationfor 60min under differenttemperatures，the samples werefetched1o detennine chit／nase activitiesand analyzethe heatandacidic—basic stabilities of chitinase．【ResultJ 1he optimumC s0I for producing chitinase with imrine h 01 stir．in was chitin and peptone was the opthnmn N source．When t}le temperature was 30℃，with 1．O％ chitin as C source and 0．1％peptoneasN$OUl~e．the produced chitinase had highestactivityandthe enzyflleactivity decreased alongwiththeincrement oftempera- ture．1heacidic-basic stabifity oftheen mwasworse，which onlyhad higheractivityin pH value of6～8．[Conclusion]1he researchlaid primaryfoun— dation for scale production of chitinase． Keywmrds