酶载体壳聚糖制备的研究.pdfVIP

化 学 工 程 师 Chemical Engineer 2008年2月 文章编号：1002—1124(2008)02—0013—02 科 研 与 酶载体壳聚糖制备的研究 开 发 杨 辉，张 剑 (武汉工业学院化学与环境工程系，湖北武汉430023) 摘 要：本文分别采用电热法、连续微波法和间歇微波法制备了几种性能不同的壳聚糖，并对产品的 脱乙酰度和分子量进行了比较。结果表明，间歇微波法制备的壳聚糖具有制备时问短、产品脱乙酰度高和 分子量大等特点，能更好地满足酶载体的要求。 关键词：酶载体；壳聚糖；脱乙酰度；粘均分子量；间歇微波法 中图分类号：0629．12 文献标识码：A Study on preparation of chitosan for the carrier of enzyme YANG Hui，ZHANG Jian (Department of Chemical＆Environmental Engineering，Wuhan Polytechnic University，Wuhan 430023，China) Abstract：Several different chitosans were prepared by using electricity heating method，continuous micro- wave heating method and interim microwave heating method，and degree of deacetylation and viscosity—average molecular weight of their products were investigated in this paper．The results showed that the chitosan prepared by interim microwave heating method has some advantage over the other two methods，such as less heating time， higher degree of deacetylation and greater viscosity—average molecular weight of the products，and it is able to further meet the requirement for the carrier of enzyme． Key words：carrier of enzyme；chitosan；degree of deacetylation；viscosity—average molecular weight；in· terim microwave heating method 壳聚糖，甲壳质经脱乙酰基得到的一种天然阳 乌氏粘度计；pHS一3C数字酸度计；家用微波炉 离子多糖，具有可降解性、较好的成膜性、良好的生 (Galanz公司)。 物相容性及一定的抗菌和抗肿瘤等优异性能，被广 甲壳素(市售)；NaOH等其他试剂均为分析纯。 泛应用于医药、食品、化工、环保等行业，素有万能 1．2 样品的制备 多糖的美誉。 1．2．1 电热法 取 10g甲壳素加到盛有 120mL 近年来在生物和食品研究方面，以壳聚糖为酶 40％NaOH溶液的烧杯中，于95％水浴锅中磁力搅 载体的研