人羊膜上皮细胞有向心肌样细胞分化的特性.pdfVIP

中国组织工程研究与临床康复第12 者第3 那 2∞8 - 01 - 15 出版 Journal 01 Clinical Rehabilitatiνe TIssue Engineering Research January 15.2008 Vol.12. No.3 @邸寰i在床医学 人羊膜上皮细胞有向心肌样细胞分化的特性炉 张路，方宁，陈代雄，刘祖林，万卫红，刘金伟，章涛 DifTer飞entiation of human amniotic epithelial cells into cardiomyocyte-like cells Zhang Lu , Fang Ni吨， Chen Dai-xiong , Liu Zu-lin , Wan Wei-hong , Liu Jin-wei , Zhang Tao Abstract AIM: To investigate 由e plasticity of human amniotic epi由elial cells into cardiomyocyte-like cells 吐Ifough 由e experiment Key Laboratory 01 ofinduαd differentiation in vilro. Cell Engineering 01 METHODS: Experiments were conducted at 由e Key Laboratory of Cell Engineering of Guizhou Province 仕om G山抽回 归。vmce， F町st Affili.ted September 2005ωDωember 2∞6. ①Six placenta samples through cesarean deliv町were coll民ted aseptically with the Hos阴阳1. Zunyi informed ∞nsents of p缸turients and the experiment was approved by the Ethics Committee of the Affiliated Hospital of Medi田1 College. Zunyi Medical College. ( Human amnion was peeled from placenta by mechanism method. A白er being rinsed with Zunyi 563∞3. D-Hanks solution，由e tissue was cut into pieces wi由 eye scissors and dig臼ted with 0.2 gIL ethylenediamine tetraacetic G山抽回 归。vmce， china acid and 0.5 gIL trypsin. 币le digested mixture was centrifugated, digested，自Itrated， and 由e fetal bovine serum was added into filter liquor to te口ninate digestion. R巳peated 由e digestion procedure for twice and combined tlrree digested cell