应用缺氧预处理建立大鼠缺血耐受模型及其特征.pdfVIP

中国组织工程研究与临床康复 第，2誊 第2筋 2008一Ol一08出版 JournalofClinicalRehabilitativeTissueEngineeringResearch January＆2008 Vo1．12，No．2 Characteristics and establishment of ischemic tolerance rat models with hypoxic preCOnditiOnin Han Xue．mei’。Gao Ming ，Xu Zhong-xin’Yang Hong’ ， 目 铒 蠢 * Abstract Department ot BACKGRoUND：We call investigate mechanism of endogenous neuroprotection in rat cerebral hypoxic tolerance tria1． Neurology, OBJECTIVE： TO observe the characteristicsof cerebralhypoxic tolerance in rat models with cerebralhypoxic Department ot preconditioning． Anesthesiology, China—Japan DESIGN：Randomized controlled observation． Friendship Hospital， SETTING：Department ofNeurology，China—Japanese Friendship Hospital，Jilin University． Jilin University, MATERIALS：The experiment was performed in the Basic Animal Experimental Center，China—Japan Friendship Hospital， Changchun 13005 1， Jilin Jilin University from ADril 2003 to April 2004．Inbred line healthy Wistar rats，of either sex，with the body mass of 200—300 g， Province．China were randomly assigned into normal control group( 6)，sham operation group( =6)，ischemic control group( 20)， hypoxic Dreconditioning(3 hours，8％O2 and 92％N2)plus ischemic group( =6O)(according to different hypoxic phases， Han Xue—mei☆ ． there were 5 time phases：30 minutes，l，3，5 and 6 hours with l2 rats in each time phase)，hypoxic preconditioning group Doctor, Associate professor, (，F 1 8)『according to different hypoxic phases，there were 3 time phases：l，3 and 5 hours wit