云南汉族人基质金属蛋白酶-1基因多态性与冠心病的相关性.pdfVIP

心脏杂志 (Chin Heart J)2008，2o(1) ·41· · 临床研究 · 云南汉族人基质金属蛋白酶．1基因多态性与冠心病的相关性 方 勤 ，王启贤 ，刘 华 ，邓东波 ，戴青原 ，张 跃 (昆明医学院第一附属医院 ：1．心内科，2．检验科，云南昆明650032) 摘要：目的 研究基质金属蛋白酶-1(matrix metalloproteinase-l，MMP-1)基因多态性与冠心病发病的关系。方法 用聚合酶链反应-限制性片段长度分析法分析经冠脉造影确诊的98例冠心病患者MMP-1启动区域-1607位点基因 型，与同期102例非冠心病患者为对照组，比较两组之间MMP-1基因多态性频率分布的差异，并结合造影情况，探 讨MMP．1基因多态性与冠脉狭窄程度的关系。结果 冠心病患者MMP-1基因2G／2G型(0，41)明显低于正常对 照者(0．56)，差异有显著性(P0．05)。2G等位基因频率在冠心病组和对照组分别为0．61、0．72(P0．05)； MMP．1基因1607．G／2G位点多态性分布与冠脉狭窄程度差异无统计学意义。结论 MMP-1基因突变型(2G／2G) 可能与冠心病遗传易感性有关。MMP-I基因-1607位点多态性与冠心病狭窄程度无关。 关键词：基质金属蛋白酶-1；基因多态性；冠状动脉疾病 中图分类号：R541．4 文献标识码：A 文章编号：1009-7236(2008)0l—D41-04 Relationship between matrix metalloproteinase-1 polymorphism and coro- nary heart disease in Han population in Yunnan FANG Qin ，WANG Qi—xian ，LIU Hua ，DENG Dong。bo ，DAI Q - n ，ZHANG Yue (1．Department of Cardiology，2．Department of Clinical Laboratory，First Affiliated Hospital，Kunming Medical College，Kunming 650032，Yunnan，China) Abstract：AIM To investigate the association between metalloproteinase-1 polymorphism and coronary heart disease(CHD)．METHODS This study was conduced with case—control design in 98 patients with angiographically documented CHD and 102 control subjects with no coronary artery disease．The genotype was determined by polymerase chain reaction-restriction fragment length polymorphism for the common-1607 allele function promoter polymorphism of MMP-1 gene．The relationship between the poly． morphism and the severity of coronary arterial stenosis was analyzed．RESULTS The result of individu． al polymorphisms analysis showed that the frequency of 2G／2G genotype in the patients(0．41)was sig． nificantly lower than that in control subjects(0．56)．The frequency of allele-1607 was respectively 0．61