夏至草醇提物对失血性休克大鼠器官一氧化氮及其合酶的影响.pdfVIP

中国微循环2008年2月第l2卷第1期 · 5· 文章编号：1007-8568(2008)O1-0005-03 · 实验研究 · 夏至草醇提物对失血性休克大鼠器官 一 氧化氮及其合酶的影响 侯亚利，赵自刚，牛春雨 ，张玉平，张 静 【摘要】 目的 观察夏至草醇提物对失血性休克大鼠器官组织一氧化氮(NO)及其合酶(NOS)的 影响。方法 Wistar雄性大鼠l8只，随机分为夏至草组、模型组和对照组(均n=6)。夏至草组和模型 组复制失血性休克模型，维持90 min，对照组仅手术。夏至草组自颈静脉缓慢推注夏至草醇提物(5 g／ ml，6 g／kg．bw)，其它两组以等量生理盐水代替。40 rain后，制备肝、肾、心肌、肺组织匀浆，检测各器官 组织匀浆NO含量及NOS活性的变化。结果 模型组肝、肾、心肌、肺组织匀浆NO含量及NOS活性均 显著高于对照组(P0．01～0．05)；夏至草组各器官组织匀浆NO含量及NOS活性显著低于模型组(P 0．01～0．05)，且各指标与对照组无统计学意义(P0．05)。结论 夏至草醇提物减轻大鼠重症失血 性休克后器官损伤的机制与降低NO的生成和释放有关。 【关键词】 夏至草；失血性休克；器官；一氧化氮 中图分类号：11285．5；R364．1；R3l1．5 文献标识码：B Effects of Ethanol Extract from Mrrubium Incisum on Nitric Oxide and Nitric Oxide Synthases of Multiple Organs in Hemorrhagic Shock Rats HOU Ya-li，ZHAO Zi·gang，NIU Chun·yu。ZHANG Yu-ping。Zhang Jing． Institute of Microcirculation，Hebei North University，Zhang,iiakou，Hebei 075029，China 【Abstract】Objective To observe the effects of ethanol extract from Mrrubium Incisum on nitric oxide (NO)and nitric oxide synthases(NOS)of organs in hemorrhagic shock rats．Methods 18 male Wistar rats were randomly divided into three groups：Mrrubium Incisum group(n=6)，model group(n=6)and control group(n=6)．The hemorrhagic shock model was established in Mrrubium Incisum group and model group for 90min．Ethanol extract from Mrrubium Incisum was infused in Mrrubium Incisum group(5g／ml，6g／kg．bw)； in other groups ethanol extract from Mrrubium Incisum was replaced by normal saline．After 3h，liver，kidney， cardiac muscle and lung were taken out for making homogenate of 10％ concentration，and the NO content and NOS activity were determined in homogenate．Results The NO content and NOS activity of liver，kidney， cardiac muscle and lung homogenate in model group were significantly increased than those of control group(P 0．01～0．05)．The NO content and NOS activ