斑马鱼反转录病毒插入突变技术的发展及其在基因饱和突变与筛选中.PDF

　遗 传 学 报 　A cta Genetica S inica , J uly 2004 , 3 1 (7) : 750～757 ISSN 0379 - 4 172 D e v e l op m e n t of Re t r o vi r u sm e di a t e d In s e r t i o n al Mu t a g e n e s i s i n Ze b r af i s h a n d It s Ap p li c a t i o n i n S a t u r a t i o n Mu t a g e n e s i s a n d Ge n e S c r e e ni n g ① Q I Fei , L IN Shuo , FAN QiChang ( College of L if e S cience , Peki ng University , B eij i ng 　10087 1 , Chi na) A b s t r a c t : The strat egie s of largescale mut agene si s and gene scre ening include chemical mut agene si s ,insertional mu t agene si s and gene trap . Insertional mut agene si s i s a method for identifying gene s by using the int e gration of DNA a s the mut agen ,thereby facilit ating the cloning of the mut at e d gene . The use of retrovirusme diat e d insertional mut agene si s in zebrafi sh ha s le d to the mut ation and rapid identification of hundre ds of gene s require d for embryonic development and cell growth . Gene trap element s are al so use d in the construction of retrovirus vector s. The whole sy st em i s likely to make zebrafi sh the fir st vert ebrat e to achieve saturation mut agene si s and gene scre ening . Ke y w or d s : zebrafi sh ; retrovirus ; insertional mut agene si s ; gene saturation mut agene si s and scre ening 斑马鱼反转录病毒插入突变技术的发展及其 在基因饱和突变与筛选中的应用 戚 　飞 , 林 　硕 , 樊启昶 ① (北京大学生命科学学院 , 北京 　10087 1) 摘 　要 : 用于大规模基因突变与筛选的主要策略有化学诱变 、插入突变 、基因诱捕 。插入突变是一种通过外源 DNA 整合的方式来获得突变体 ,并克隆得到对应突变