钛表面羟基磷灰石涂层含镧量对附着细胞生物学性能的影响.doc

钛表面羟基磷灰石涂层含镧量对附着细胞生物学性能的影响 唐霞1，孟玉坤2 (1.重庆医科大学附属口腔医院 修复科，重庆 400015； 2.四川大学华西口腔医学院 修复科，四川 成都 610041) 摘要 目的：旨在研究钛表面羟基磷灰石涂层含镧量对附着细胞生物学性能的影响A 作者简介 唐霞1982年生，女，重庆人，硕士，重庆医科大学附属口腔医院修复科 重庆 400000，电话：023Email:tx.1124@163.com HA coated Ti surface with different La contents and study of its cytocompatibility TANG Xia1,MENG Yu-kun2(1. Dept. of Prosthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China; 2. Dept. of Prosthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China) Abstract Objective: To investigate the cytocompatibility of the lanthanum-doped hydroxyapatite (HA) coating formed on Titanium (Ti) surface after microarc oxidation, So as to provide theoretical support for the promotion of surface treatment technique of pure Ti. Method: Pure Ti plates were coated by means of microarc-oxidation in solutions cotaining 0g/l, 0. 3g/l, 0.8g/l La, thus HA coated Ti surface with different La contents were obtained. Specimens were seeded with bovine serum albumin. The initial adhesion of protein on the surface was tested by means of bicinchoninic acid (BCA) protein analyzing. Ti specimens with different surface coatings were also seeded with osteoblasts (OB) and cultured in the osteogenic medium. The proliferation and differentiation of OB were evaluated by means of MTT assay, alkaline phosphatase activity analysis, and scanning electronic microscopic observations. Result: Ti specimens in 0g/l La and 0.3g/l La groups could accelerate the early adhesion and spreading of OB on the Ti surfaces. The SEM observation showed that the cells grew well with the Ti specimens and compacted tightly for the groups oxidized in 0g/l La and 0.3g/l La contents. The Ti surfaces of these two groups promoted the cells proliferation, and cells grew in a multilayer manner. The modified Ti surfaces increased the ALP activity especially on the specimens oxidized in solution with 0g/l La and 0.3g/l La contents. The