assessment of 5-ht7 receptor agonists selectivity using nociceptive and thermoregulation tests in knockout versus wild-type mice评估5-ht7受体受体激动剂选择性使用基因敲除与野生型小鼠痛觉和温度调节测试.pdfVIP

Hindawi Publishing Corporation Advances in Pharmacological Sciences Volume 2012, Article ID 312041, 9 pages doi:10.1155/2012/312041 Research Article Assessment of 5-HT7 Receptor Agonists Selectivity Using Nociceptive and Thermoregulation Tests in Knockout versus Wild-Type Mice Alex Brenchat,1 Maria Rocasalbas,1 Daniel Zamanillo,1 Michel Hamon,2 ´ 1 1 Jose Miguel Vela, and Luz Romero 1 Department of Pharmacology, Drug Discovery and Preclinical Development, ESTEVE, Avenida Mare de Deu de Montserrat 221, ´ 08041 Barcelona, Spain 2 UMR 894 INSERM-CPN/UPMC, Faculte de Medecine Pierre et Marie Curie, Site Pitie-Salpetriere, 91 boulevard de l’Hopital, ´ ´ ´ ˆ ` ˆ 75634 Paris Cedex 13, France Correspondence should be addressed to Luz Romero, lromero@esteve.es Received 17 February 2012; Accepted 27 April 2012 Academic Editor: Karim A. Alkadhi Copyright © 2012 Alex Brenchat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. No study has ever examined the effect of 5-HT7 receptor agonists on nociception by using 5-HT7 receptor knockout mice. Basal sensitivity to noxious heat stimuli and formalin-induced nociception in both phase I and II of the formalin test did not differ in 5- HT7 receptor knockout mice and paired wild-type controls. Similarly, there was no significant difference in basal body temperature betwee