灵芝孢子粉在早期糖尿病大鼠视网膜抗氧化反应中作用.doc

灵芝孢子粉在早期糖尿病大鼠视网膜抗氧化反应中作用摘 要：目的：探讨灵芝孢子粉对早期实验性糖尿病视网膜病变(diabetic retinopathy，DR) 的作用及其机理。方法：用链脲佐菌素(streptozotocin，STZ) 诱发糖尿病大鼠模型，并以高糖高脂饮食。实验鼠被随机分为对照组、模型组、治疗组。治疗组用灵芝孢子粉灌胃，模型组和对照组用等容量生理盐水灌胃。连续3个月后观察大鼠视网膜中脂质过氧化终产物丙二醛(malondialdehyde ，MDA) 、超氧化物歧化酶(superoxide dismutase ，SOD) 及大鼠血清中一氧化氮(nitrogen monoxidum NO) 、一氧化氮合酶(nitricoxide synthase，NOS)含量的变化。结果：灵芝孢子粉有降低视网膜中MDA含量，提高视网膜中SOD活性的作用，并能提高血清中NO、NOS水平，与模型组比较差异有显著性。结论：灵芝孢子粉能增强糖尿病大鼠抗氧化能力，减轻视网膜的氧化损伤，从而对糖尿病视网膜病变起一定保护作用。 关键词：灵芝孢子粉；糖尿病视网膜病变；抗氧化 中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2008)03-0637-02 Effects of Ganoderma Lucidum Spores on Antioxidatizing Reaction in the Retinal Tissue of Diabetic Rats YUAN Yan-xia，WANG Shu-qiu (The Basic Medical College of Jiamusi University，Jiamusi 154007，Heilongjiang，China) Abstract：Objective：To study the therapeutic effect and mechanism of Ganoderma lucidum spores on early experimental diabetic retinopathy. Methods：Diabetic rats induced by streptozotocin(STZ) and gived high lipin and carbohydrate food and drink.The rats were randomly divided into 3 groups：normal group，model group，treated group.They were treated with Ganoderma lucidum spores in the treated group，and the rats were fed normal saline into stomachs in the normal group and model group. For at 3 months the activity of malondialdehyde (MDA) and superoxide dismutase (SOD) in retinal tissue，the content of nitrogen monoxidum(NO) and nitricoxide synthase(NOS)in serum. Results：Compared with the model group，the content of MDA in retina was decreased evidently，the activity of SOD in retina and the content of NO and NOS in serum were increased distinctly in treated groups with Ganoderma lucidum spores，there was a statistic significant difference between them (P0.05) 。提示灵芝孢子粉对糖尿病大鼠血糖与体重有明显改善作用。糖尿病大鼠血糖均保持16.7mmol/L以上，部分大鼠在注射后1天～1周的时间内出现突然死亡，皆删除。 2.2 各组指标变化及比较 见表2。糖尿病组大鼠视网膜中MDA含量高于对照组，经灵芝孢子粉治疗后MDA含量下降，差异具有显著性(P0.05)。 3讨 论 本实验中，糖尿病大鼠视网膜MDA含量明显增高，视网膜中的SOD含量和血清中的NO、NOS活性都显著降低，说明糖尿病大鼠机体抗氧化能力降低，视网膜亦受到氧化损伤。灵芝孢子粉组能明显的降低血糖，显著降低视