bone marrow increases human islets insulin positive cells in co-culture quantification with flow cytometry骨髓增加人类胰岛胰岛素阳性细胞共培养与流式细胞术定量.pdfVIP

bone marrow increases human islets insulin positive cells in co-culture quantification with flow cytometry骨髓增加人类胰岛胰岛素阳性细胞共培养与流式细胞术定量.pdf

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bone marrow increases human islets insulin positive cells in co-culture quantification with flow cytometry骨髓增加人类胰岛胰岛素阳性细胞共培养与流式细胞术定量

Vol.1, No.4, 109-117 (2011) Journal of Diabetes Mellitus doi:10.4236/jdm.2011.14015 Bone marrow increases human islets insulin positive cells in co-culture: quantification with flow cytometry 1 1 1 1,2 1* Zhengke Wang , Fang Xiong , Mary Hassani , John Z. Q. Luo , LuGuang Luo 1Center for Stem Cell Biology, Roger Williams Hospital, Providence, USA; *Corresponding Author: Lluo@ 2Alpert Medical School, Brown University, Providence, USA. Received 30 August 2011; revised 4 October 2011; accepted 15 October 2011. ABSTRACT results in a significant loss of structural integrity within the islets and a decline in glucose-stimulated insulin re- We have previously demonstrated that allogene- lease [6-8]. ic human bone marrow (BM) supports human Our previous studies have indicated that bone marrow islet function and longevity in vitro. We hypoth- can maintain β cell function by evaluating insulin secre- esize that BM supporting human islets may incl- tion even after 200 days of in vitro culture [9]. Explora- ude to increase β-cell in cultured islets. In this tion of mechanisms how BM support human islets in study, we developed a method to quantify insu-

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