characterization of a lipoyl domain-independent b-cell autoepitope on the human branched-chain acyltransferase in primary biliary cirrhosis and overlap syndrome with autoimmune hepatitis描述的lipoyl特定领域b细胞autoepitope人类支链酰基转移酶在原发性胆汁性肝硬化和自身免疫性肝炎重叠综合征.pdfVIP

Clinical Developmental Immunology, June–December 2003 Vol. 10 (2–4), pp. 173–181 Characterization of a Lipoyl Domain-independent B-cell Autoepitope on the Human Branched-chain Acyltransferase in Primary Biliary Cirrhosis and Overlap Syndrome with Autoimmune Hepatitis a,b,c a a a a ANTAL CSEPREGI , PETRA OBERMAYER-STRAUB , SUSANNE KNEIP , ANNE KAYSER , STEPHANIE LOGES , a ´ ´ b d a ELEONORE SCHMIDT , ELEMER NEMESANSZKY , FERENC SZALAY , MICHAEL P. MANNS and CHRISTIAN P. STRASSBURGa,* aDepartment of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Carl-Neuberg-Street 1, 30625 Hannover, Germany; bDepartment of Gastroenterology and Medicine, Polyclinic of the Hospitaller Brothers of St. John of God in Budapest Germany; cDepartment of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University, Magdeburg, Germany; d1st Department of Medicine, Semmelweis Medical University, Budapest, Hungary Background and aims : Antimitochondrial antibodies (AMA) which recognize pyruvate acetyltransferase (PDC-E2) represent a highly diagnostic feature of primary biliary cirrhosis (PBC). The analysis of immunofluorescence (IF)-AMA-positive sera in PBC patients indicates a conformational epitope located within the lipoyl binding domain of bovine branched-chain acyltransferase (BCKADC-E2) alone or in combination with AMA