病理性瘢痕成纤维细胞中连接蛋白43免疫电镜观察.docVIP

病理性瘢痕成纤维细胞中连接蛋白43免疫电镜观察[摘要]目的：观察连接蛋白43（Connexin43,Cx43）在病理性瘢痕成纤维细胞中的表达，探讨Cx43及其构成的缝隙连接在病理性瘢痕中的调控作用。方法：取正常皮肤2例、正常修复组织2例、增生性瘢痕5例、瘢痕疙瘩5例，用特异性抗体（Cx43）作为标记物,用胶体金标记的IgG作示踪物进行免疫电镜观察Cx43的分布与表达，并对其进行定位, 同时观察不同标本成纤维细胞的超微结构变化。结果：Cx43在正常修复组织及正常皮肤成纤维细胞的胞浆及近胞膜上表达，且明显集中于粗面内质网；Cx43在增生性瘢痕成纤维细胞的表达明显减少，粗面内质网中见到少许Cx43金颗粒的聚集；瘢痕疙瘩成纤维细胞仅在胞浆中见到少许Cx43散在金颗粒。结论：成纤维细胞Cx43的表达下调可能是造成病理性瘢痕组织中成纤维细胞间缝隙连接细胞间通讯异常，从而导致病理性瘢痕发生的因素之一。 [关键词]连接蛋白43；病理性瘢痕；免疫电镜 [中图分类号]R619+.6 [文献标识码]A [文章编号]1008-6455（2009）01-0063-03 Immunoelectron-microscopy observation of Cx43 in fibroblasts of pathological scar ZHANGJuan-juan1,LV Shi-jun2 (1.Department ofStomatological Histopathology; 2. Department ofPathology, Weifang Medical Collage, Weifang 261042,Shandong,China) Abstract:ObjectiveTo investigate the role of connexin43 and gap junctional intercellular communication composed of it in pathological scar by studying expression of connexin43 in fibroblasts.MethodsThe expression, distribution and orientation of Cx43 were observed in 5 hypertrophic scars,5 keloids,2 normal repaired tissues,2 normal skin samples by immunoelectron-microscopy with special antibody for marker and gold colloidal labelled IgG for tracer. The ultrastructure of fibroblasts were also observed.ResultsIn normal skin and repaired tissues, Cx43 were largely expressed at endochylema, cell membrane, and concentrated at the rough endoplasmic reticulum. It was clearly reduced in hypertrophic scars, a few Cx43 granules were observed at the rough endoplasmic reticulum. There were hardly Cx43 granules in keloids.ConclusionLower expression of Cx43 in the fibroblasts maybe one reason of leading to pathological scar. It has a correlation between Cx43 and the abnormality of gap junctional communication channel and Cx43 may have important function in the regulation of pathological scar. Key words: connexin43; pathological scar; immunoelectron-microscopy 增生性瘢痕(hypertrophic scar, HS)和瘢痕疙瘩(keloid, K)统称为病理性瘢痕，是皮肤对创伤、炎症、烧伤、外科或自发的一种过度的皮肤纤维增生性疾病，为当今医学研