cloning and expression of randomly mutated bacillus subtilis -amylase genes in hb101克隆和表达的随机突变枯草芽孢杆菌在hb101淀粉酶基因.pdfVIP

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cloning and expression of randomly mutated bacillus subtilis -amylase genes in hb101克隆和表达的随机突变枯草芽孢杆菌在hb101淀粉酶基因.pdf

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cloning and expression of randomly mutated bacillus subtilis -amylase genes in hb101克隆和表达的随机突变枯草芽孢杆菌在hb101淀粉酶基因

SAGE-Hindawi Access to Research Biotechnology Research International Volume 2011, Article ID 305956, 5 pages doi:10.4061/2011/305956 Research Article Cloning and Expression of Randomly Mutated Bacillus subtilis α-Amylase Genes in HB101 Mohammed Rabbani,1, 2 Hamid MirMohammad Sadeghi,1 Fatemeh Moazen,1 Mehran Rahimi,3 and Golnaz Salehi3 1 Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Sciences Research Centre, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran 2 Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran 3 Isfahan Pharmaceutical Sciences Research Centre, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran Correspondence should be addressed to Mohammed Rabbani, rabanim@ Received 12 April 2011; Accepted 26 May 2011 Academic Editor: Triantafyllos Roukas Copyright © 2011 Mohammed Rabbani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The aim of this study was to isolate and express the randomly mutated α-amylase gene from B. subtilis strain 168. BS168F: 5 - gtgtcaagaatgtttgc-3 and BS168R: 3 -gttttgttaaaagatga-5 primers were used to amplify the amylase gene using the following cycle ◦ ◦