d-aminoacid oxidase-fe2o3 nanoparticle complex mediated antitumor activity in swiss albino miced-aminoacid oxidase-fe2o3纳米颗粒复杂介导抗肿瘤活性在瑞士白化病老鼠.pdfVIP

Journal of Cancer Therapy, 2011, 2, 666-674 doi:10.4236/jct.2011.25089 Published Online December 2011 (http://www.SciRP.org/journal/jct) D-Aminoacid Oxidase-Fe O Nanoparticle 2 3 Complex Mediated Antitumor Activity in Swiss Albino Mice 1 2 2 3* Saratchandran A. Divakaran , K. M. Sreekanth , K. V. Rao , Cherupally Krishnan Krishnan Nair 1Amala Cancer Research Centre, Amala Nagar, Thrissur, India; 2Department of Materials Science, Tmfy-MSE, Royal Institute of Technology, Stockholm, Sweden; 3Pushpagiri Institute of Medical Sciences Research Centre, Thiruvalla, India. * E-mail: ckknair@ th st th Received October 20 , 2011; revised November 21 , 2011; accepted December 5 , 2011. ABSTRACT D-Aminoacid oxidase (DAO) was isolated from fresh porcine kidney; its cytotoxic potential was studied under in vitro and in vivo conditions. The isolated DAO was complexed with Fe O nanoparticles and its potential as an oxidation 2 3 therapeutic agent was analysed. The ability of the complex in eliciting H O mediated cytotoxicity was studied on Dal- 2 2 ton’s lymphoma ascites cells (DLA). The induction of apoptosis in DLA cells by Fe O -DAO complex was studied by 2 3 morphological examination and alkaline single cell gel electrophoresis (comet assay). The antitumor activity of the complex was investigated by oral administration of the comp