definition of the gene content of the human genome the need for deep experimental verification人类基因组的基因内容的定义需要实验验证.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2001; 2: 169–175. DOI: 10.1002/cfg.81 Review Article Definition of the gene content of the human genome: the need for deep experimental verification Andrew J. G. Simpson*, Sandro J. de Souza, Anamaria A. Camargo and Ricardo R. Brentani The Ludwig Institute for Cancer Research, Rua Professor Antoˆnio Prudente, 109, 4th floor, Sa˜o Paulo, 01509-010, SP, Brazil Abstract *Correspondence to: A. J. G. Simpson, The Ludwig Based on the analysis of the drafts of the human genome sequence, it is being speculated Institute for Cancer Research, Rua that our species may possess an unexpectedly low number of genes. The quality of the Professor Antonio Prudente, 109, drafts, the impossibility of accurate gene prediction and the lack of sufficient transcript 4th floor, Sa˜o Paulo, 01509-010, SP, Brazil. sequence data, however, render such speculations very premature. The complexity of E-mail: asimpson@.br human gene structure requires additional and extensive experimental verification of transcripts that may result in major revisions of these early estimates of the number of human genes. Copyright # 2001 John Wiley Sons, Ltd. Received: 4 April 2001 Accepted: 5 April 2001 Keywords: human genome; genes; DNA sequence; gene prediction; transcriptome; ESTs Introduction has entailed, has not led to the most eagerly awaited portion of the information that it contains, the Of all the justifications to sequence the human identification of human genes. The