detection of circulating tumor cells (ctcs) in patients with lung carcinoma by real-time fluorescent quantitative-pcr approach before and after chemotherapy检测循环肿瘤细胞(ctc)在肺癌患者化疗前后实时荧光定量pcr方法.pdfVIP

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detection of circulating tumor cells (ctcs) in patients with lung carcinoma by real-time fluorescent quantitative-pcr approach before and after chemotherapy检测循环肿瘤细胞(ctc)在肺癌患者化疗前后实时荧光定量pcr方法.pdf

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detection of circulating tumor cells (ctcs) in patients with lung carcinoma by real-time fluorescent quantitative-pcr approach before and after chemotherapy检测循环肿瘤细胞(ctc)在肺癌患者化疗前后实时荧光定量pcr方法

Vol.1, No.3, 231-238 (2009) Health doi:10.4236/health.2009.13038 Detection of circulating tumor cells (CTCs) in patients with lung carcinoma by real-time fluorescent quantitative-PCR approach before and after chemotherapy 1 1 2 1 1 1 Ming-Jian Ge , Qing-Chen Wu , Mei Wang , Li Li , Xiao-Long Zhao , Qiao-Min Huang , Liang-Bin Li1 1Department of Thoracic Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing, China mingjian_ge@ 2Department of Clinical Laboratory, the First Affiliated Hospital, Chongqing Medical University, Chongqing, China Received 18 September 2009; revised 9 October 2009; accepted 10 October 2009. ABSTRACT P=0.001).The positive rate before chemotherapy was 71.9% (23/32), while that after chemotherapy Circulating tumour cells (CTCs) are referred to the was 37.5% (12/32), indicating that 11 patients con- tumour cells that disseminated from the primary verted into negative after chemotherapy. Of the 16 tumour and survive in circulating during the pro- patients which were in Ⅳ-stage, 11 cases were po- ceeding of tumour growth. As surgical treatment sitive (11/16,68.8%). Surprisingly, of the remaining evolves and local control has improved, the failure 16 patients which were Ⅱ/Ⅲ stage, 12 cases were of cancer treatment has largely remained the re- regarded as positive according to the criteria sult of systemic metastasis. Selection of patients