原创力文档detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (rt-lamp)古典猪瘟病毒核酸的检测逆转录loop-mediated等温扩增(rt-lamp).pdfVIP
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detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (rt-lamp)古典猪瘟病毒核酸的检测逆转录loop-mediated等温扩增(rt-lamp)
Vol.3, No.7, 447-452 (2011) Health
doi:10.4236/health.2011.37074
Detection of nucleic acid of classical swine fever virus
by reverse transcription loop-mediated isothermal
amplification (RT-LAMP)
1 2 3 1*
Kanokwan Wongsawat , Tararaj Dharakul , Phairot Narat , Jundee Rabablert
1Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand;
*Correspondence author: jundee@su.ac.th
2Department of Immunology, Faculty of Medicine, Siriraj Hospital Mahidol University, Bangkok, Thailand;
3Bangkok Agro-Industrial Products Public Co., Ltd., Bangkok, Thailand.
Received 2 May 2011; revised 31 May 2011; accepted 27 June 2011.
ABSTRACT 5’UTR functions as an internal ribosomal entry site for
translation initiation of the pre-polyprotein and genome
Classical swine fever virus (CSFV) is the causa-
replication [3]. The host range of CSFV is narrow; this
tive agent of Classical swine fever which is a
virus is restricted to its natural hosts, domestic pigs and
highly contagious disease affecting swine and
feral pigs. The control of CSF is based on stamping out
resulting in severe economic losses. In this
policies and/or on vaccination. However, failed immu-
study, we developed reverse transcription loop-
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