determination of clopidogrel carboxylic acid in human plasma by lc-msms在人血浆测定氯吡格雷羧酸lc-msms.pdfVIP

American Journal of Analytical Chemistry , 2011, 2, 447-455 doi:10.4236/ajac.2011.24054 Published Online August 2011 (http://www.SciRP.org/journal/ajac) Determination of Clopidogrel Carboxylic Acid in Human Plasma by LC-MS/MS 1 2 3 Mohamed El-Husseiny El-Sadek , Samia Mahmoud Moustafa , Hussien Omar Kadi , Abdul Moneim Ali Al-Hakami2 1 Faculty of Pharmacy, Zagazig University, Zagazig, Egypt 2Faculty of Pharmacy, Suez Canal University, Suez, Egypt 3Faculty of Medicine, Sana ’a University, Sana ’a, Yemen E-mail : alhakami2@ Received April 2, 2011; revised May 12, 2011; accepted May 19, 2011 Abstract Background: Clopidogrel, is a thienopyridine derivative labeled for use to prevent thrombosis after coro- nary artery stenting. Pharmacokinetics of clopidogrel is studied indirectly by quantification of carboxylic acid which is a major metabolite of Clopidogrel. Objective: The aim of this work is to develop and validate a rapid, simple and sensitive LC/MS/MS assay method for the determination of Clopidogrel carboxylic acid in human plasma using Clopidogrel-D4-carboxylic acid as internal standard. Methods: Analytes was ex- tracted from 200 μl of plasma by a simple liquid-liquid extraction using diethyl ether – n-hexane (80:20, v/v). The chromatographic separations were achieved on a C18 column using Methanol, de-ionized water and formic acid as a mobile phase at flow rate of 0.5 ml/minute. Analysis was monitored by multiple re