原创力文档determination of phytosterols in oryza sativa l. cultivars by liquid chromatography atmospheric chemical ionization time-of-flight mass spectrometer (lc-apci-tof-ms) coupled with a modified quechers method英文论文.pdfVIP
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determination of phytosterols in oryza sativa l. cultivars by liquid chromatography atmospheric chemical ionization time-of-flight mass spectrometer (lc-apci-tof-ms) coupled with a modified quechers method英文论文
Vol.1, No.1, 15-19 (2012) Journal of Agricultural Chemistry and Environment
/10.4236/jacen.2012.11003
Determination of phytosterols in Oryza sativa L.
cultivars by liquid chromatography atmospheric
chemical ionization time-of-flight mass spectrometer
(LC-APCI-TOF-MS) coupled with a modified
QuEChERS method
* * #
Seung Rok Lee , Dong Cheon Yun , Seung Ho Lee, Si Young Yang, In Seon Kim
Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life
Sciences, Chonnam National University, Gwangju, South Korea; #Corresponding Author: mindzero@chonnam.ac.kr.
Received 3 September 2012; revised 15 October 2012; accepted 28 October 2012
ABSTRACT 1. INTRODUCTION
Development of analytical methods for the de- Phytosterols have shown to function as biologically ac-
termination of phytosterols in whole grains is tive substrates that contribute to reduce the risk of hu-
one of growing interests due to their clinical and man diseases [1,2]. Biological functions of phytosterols
nutritional functions in human. In this study, we have been demonstrated by their cholesterol-reducing pro-
examined simultaneous determination of β-si- perties and anticancer effects [3,4]. Typical representa-
tosterol, stigmasterol and campesterol in Korean tives of phytosterols found commonly in plants [5] in-
rice cultivars by liquid chromatography atmos- clude sitosterol, stigmasterol and campesterol (Figure 1).
pheric chemical ionization time-of-flight mass They have
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