development and validation of a stability-indicating hptlc method for analysis of rasagiline mesylate in the bulk drug and tablet dosage form开发和验证stability-indicating效果方法分析rasagiline甲磺酸的原料药和片剂剂型.pdfVIP

Hindawi Publishing Corporation Chromatography Research International Volume 2012, Article ID 273604, 6 pages doi:10.1155/2012/273604 Research Article Development and Validation of a Stability-Indicating HPTLC Method for Analysis of Rasagiline Mesylate in the Bulk Drug and Tablet Dosage Form Singaram Kathirvel,1 Suggala Venkata Satyanarayana,2 and Garikapati Devalarao3 1 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur 522002, India 2 Department of Chemical Engineering, JNTU College of Engineering, Anantapur 515002, India 3 Department of Pharmaceutical Analysis, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada 520008, India Correspondence should be addressed to Garikapati Devalarao, devalarao2007@ Received 2 May 2011; Revised 18 July 2011; Accepted 2 October 2011 Academic Editor: Wenkui Li Copyright © 2012 Singaram Kathirvel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A simple and sensitive thin-layer chromatographic method has been established for analysis of rasagiline mesylate in pharma- ceutical dosage form. Chromatography on silica gel 60 F254 plates with 6 : 1 : 2(v/v/v) butanol-methanol water as mobile phase furnished compact spots at Rf 0.76 ±0.01. Densitometric analysis was performed at 254 nm. To show the specificity of the method, rasagiline mesylate was subjected to acid, base, neutral hydrolysis, oxidation, photolysis, and thermal decomposition, and the peaks of degradation products were well resolved from that of the pure drug. Linear regression analysis revealed a good linear relationship between peak area and amount of