development and validation of rp-hplc method for quantitative estimation of vinpocetine in pure and pharmaceutical dosage forms产物的开发和验证方法,定量估计长春西汀的纯和制药剂型.pdfVIP

SAGE-Hindawi Access to Research Chromatography Research International Volume 2011, Article ID 801656, 7 pages doi:10.4061/2011/801656 Research Article Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms Subrata Bhadra,1 Sreedam Chandra Das,1 Sumon Roy,1 Shamsul Arefeen,2 and Abu Shara Shamsur Rouf3 1 Department of Pharmacy, Southeast University, Banani, Dhaka 1213, Bangladesh 2 Product Development, ACI Limited, Narayanganj 1400, Bangladesh 3 Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh Correspondence should be addressed to Subrata Bhadra, subrata1011@ Received 14 June 2011; Revised 16 August 2011; Accepted 29 August 2011 Academic Editor: Esther Turiel Copyright © 2011 Subrata Bhadra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A simple, precise, specific, and accurate reversed phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for determination of vinpocetine in pure and pharmaceutical dosage forms. The different analytical performance parameters such as linearity, accuracy, specificity, precision, and sensitivity (limit of detection and limit of quantitation) were determined according to International Conference on Harmonization ICH Q2 (R1) guidelines. RP-HPLC was conducted on Zorbax C18 (150 mm length × 4.6 mm ID, 5 μm) column. The mobile phase was consisting of buffer (containing 1.54% w/v ammonium acetate solution) and acetonitrile in the ratio (40 : 6