原创力文档development and validation of an hplc method for simultaneous determination of nine active components in ‘da-chai-hu-tang’开发和验证的高效液相色谱法同时测定9活性成分的u201cda-chai-hu-tangu201d.pdfVIP
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development and validation of an hplc method for simultaneous determination of nine active components in ‘da-chai-hu-tang’开发和验证的高效液相色谱法同时测定9活性成分的u201cda-chai-hu-tangu201d
Chinese Medicine, 2011, 2, 20-28
doi:10.4236/cm.2011.21004 Published Online March 20 11 (http://www.SciRP.org/journal/cm)
Development and Validation of an HPLC Method for
Simultaneous Determination of Nine Active Components
in ‘Da-Chai-Hu-Tang’
Lingli Zheng, Deshi Dong*
The First Affiliated Hospital, Dalian Medical University , Dalian , China
E-mail: Zheng_ll2009@126.com
Received December 21, 2010; revised January 25, 2011; accepted February 2, 2011
Abstract
In this study, a simple, reliable and accurate method for the simultaneous separation and determination of
naringin, hesperidin, neohesperidin, baicalin, wogonoside, baicalein, wogonin, emodin and chrysophanol in
‘Da-Chai-Hu-Tang’ was developed by reverse-phase high-performance liquid chromatography (RP-HPLC).
The chromatographic separation was performed on an Agilent ZORBAX C18 column (250 mm × 4.6 mm
i.d., 5.0 μm), and the mobile phase composed of methanol and water containing 1% (v/v) acetic acid was
used to elute the targets in a gradient elution mode. The flow rate and detection wavelength were set at 0.8
ml/min and 280 nm, respectively. All calibration curves of the nine components expressed good linearities
(r2 ≥ 0.9992) within the tested ranges. The RSD values demonstrated the intra- and inter-day precisions were
less than 2.89%, and the recoveries of the investigated compounds were between 96.22% and 105.28%. The
proposed method is simple, precise, specific, sensitive, and successfully applied to determine the nine marker
compounds in ‘Da-Chai-Hu-Tang’ for quality control.
Keywords: High-Performance Liquid Chromatography, ‘Da-Chai-Hu-Tang’, Traditional Chinese Medicine,
Multiple Compounds Determination
1. Introduction p
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