development of a high-throughput cell based 384-well influenza a quantification assay for interpandemic and highly pathogenic avian strains开发基于高通量细胞384 -流感interpandemic量化分析和高致病性禽流感毒株.pdfVIP

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development of a high-throughput cell based 384-well influenza a quantification assay for interpandemic and highly pathogenic avian strains开发基于高通量细胞384 -流感interpandemic量化分析和高致病性禽流感毒株.pdf

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development of a high-throughput cell based 384-well influenza a quantification assay for interpandemic and highly pathogenic avian strains开发基于高通量细胞384 -流感interpandemic量化分析和高致病性禽流感毒株

Vol.2, No.1, 32-37 (2010) Health doi:10.4236/health.2010.21006 Development of a high-throughput cell based 384-well influenza A quantification assay for interpandemic and highly pathogenic avian strains Melicia R. Gainey, Ann M. Wasko, Jennifer N. Garver, David J. Guistino, Eric M. Vela*, John E. Bigger Battelle Biomedical Research Center West Jefferson, OH 43162, USA; velae@ Received 25 November 2009; revised 1 December 2009; accepted 2 December 2009. ABSTRACT give rise to a pandemic. Given the economic and health implications of these viral strains, many new vaccines Influenza remains a world wide health threat, and therapeutics are being developed to counter this thus the need for a high-throughput and robust threat. In order to determine efficacy of these novel assay to quantify both seasonal and avian in- products, it is necessary to quantify antibody responses fluenza A strains. Therefore, a 384-well plate and influenza A viruses in various matrices, ideally in a format was developed for the median tissue high-throughput format. Currently, several high throu- culture infectious dose assay (TCID50) utilizing ghput assays to detect influenza virus exist which utilize the detection of nucleoprotein by an in situ en- reverse transcription polymerase chain reaction (RT-PCR) zyme linked immunosorbent assay (ELISA) alone [3], and coupled with flow cytometery [4] or utilize which was optimized for sensitivity in this assay. a latex turbid