development of a novel protein multi-blotting device开发一种新型蛋白质multi-blotting设备.pdfVIP

J. Biomedical Science and Engineering, 2010, 3, 1125-1132 JBiSE doi:10.4236/jbise.2010.312146 Published Online December 2010 (http://www.SciRP.org/journal/jbise/). Development of a novel protein multi-blotting device Amin M. Hagyousif1 2 3 1 , Voon J. Chong , Hiroki Yokota , Stanley Y. P. Chien 1Department of Electrical and Computer Engineering, Indiana University Purdue University Indianapolis, Indianapolis, USA; 2Department of Biomedical Engineering, Indiana University Purdue University Indianapolis, Indianapolis, USA; 3Department of Biomedical Engineering, Rensselaer Polytechnic Institute, New York, USA Email: schien@ Received 10 Octorber 2010; revised 18 Octorber 2010; accepted 20 Octorber 2010. ABSTRACT lized to a positively-charged membrane [2-4]. Using primary antibodies specific to the target proteins as well Blotting is a common technique widely used for mo- as secondary antibodies for signal amplification and vi- lecular analysis in life sciences. The Western blot, in sualization, expression and modification of the target particular, is a process of transferring protein sam- proteins can be investigated. Although the procedure is ples from a polyacrylamide gel to a blotting mem- well established and many tools are available for gel brane and detecting the levels of specific proteins fractionation and blotting, one of the bottlenecks is its through reactions with primary and secondary anti- limited