development of a molecular marker to identify a candidate line of turmeric (curcuma longa l.) with a high curcumin content开发的分子标记识别候选的姜黄(姜黄l .)与姜黄素含量很高.pdfVIP

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development of a molecular marker to identify a candidate line of turmeric (curcuma longa l.) with a high curcumin content开发的分子标记识别候选的姜黄(姜黄l .)与姜黄素含量很高.pdf

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development of a molecular marker to identify a candidate line of turmeric (curcuma longa l.) with a high curcumin content开发的分子标记识别候选的姜黄(姜黄l .)与姜黄素含量很高

American Journal of Plant Sciences , 2011, 2, 15-26 15 doi:10.4236/ajps.2011.21002 Published Online March 2011 (http://www.SciRP.org/journal/ajps) Development of a Molecular Marker to Identify a Candidate Line of Turmeric (Curcuma longa L.) with a High Curcumin Content —Development of Molecular Marker of Turmeric 1,2 2 2,3 4 2 Hiroshi Hayakawa , Tetsuya Kobayashi , Yukio Minaniya , Katsura Ito , Akira Miyazaki , 2* 2 Tatsuya Fukuda , Yoshinori Yamamoto 1United Graduate School of Agricultural Sciences, Ehime University, Monobe, Nankoku, Japan; 2Faculty of Agriculture, Kochi Uni- versity, Monobe, Nankoku, Japan; 3Graduate School of Integrated Arts and Sciences, Kochi University, Monobe, Nankoku, Japan; 4JST Innovation Satellite Kochi, Laboratory of Applied Entomology, Faculty of Agriculture, Kochi University, Monobe, Nankoku, Japan. Email: tfukuda@kochi-u.ac.jp Received October 13th, 2010; revised November 15th, 2010; accepted November 29th, 2010. ABSTRACT Dried and fresh rhizomes of the spice turmeric (Curcuma longa L.) are well known in traditional medicine, and curcu- min is widely used in various geographic regions. Although there are differences in the amount of curcumin within this species, identification of the candidate line by rhizome is difficult because of the relative simplicity of its morphological characteristics. To accurately identify lines of C. longa with a high content of curcumin, we analysed several sequences of chloroplast DNA. First, to determine the appropriate outgroup taxa in which to conduct infraspecific analyses of C. longa, we reconstructed the molecular phylogenetic tree of C. longa and its allied species. The results showed that C.