原创力文档detection, identification and characterization of staphylococci in street vend foods—characterization of staphylococcus isolates葡萄球菌的检测、识别和描述在街上出售foods-characterization葡萄球菌分离株.pdfVIP
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detection, identification and characterization of staphylococci in street vend foods—characterization of staphylococcus isolates葡萄球菌的检测、识别和描述在街上出售foods-characterization葡萄球菌分离株
Food and Nutrition Sciences, 2011, 2, 304-313
doi:10.4236/fns.2011.24044 Published Online June 2011 (/journal/fns)
Detection, Identification and Characterization of
Staphylococci in Street Vend Foods
—Characterization of Staphylococcus Isolates
Yadahalli Shrihari Rohinishree, Pradeep Singh Negi
Human Resource Development, Central Food Technological Research Institute, Mysore, India.
Email: psnegi@cftri.res.in
Received January 13th, 2011; revised March 28th, 2011; accepted April 7th, 2011.
ABSTRACT
In the present investigation the diversity of the Staphylococcus species in different street vend food samples was studied.
A total of 35 staphylococcal food isolates comprising of various species from 14 different street vend food samples were
identified and characterized phenotypically. Staphylococcus aureus was found to be the most prevalent species in these
foods. A PCR-RFLP analysis based on 16S rRNA gene was used for identification of Staphylococcus species. Isolates
showing distinct RFLP pattern for AluI restriction digestion were selected for nucleotide sequence analysis. Phyloge-
netic tree constructed using the multiple alignments of 16S rRNA gene sequences of isolates showed a hotspot region of
169 bp and the relationship among species was evaluated by bootstrap values generated in phylogenetic analysis. 16S
rRNA gene sequences allowed bacterial identifi cation that was reproducible and more accurate than that obtained by
phenotypic testing . 16S rRNA gene sequence analysis would be helpful in timely and correct identification of pathogens.
Keywords : Staphylococcus, 16S rRNA Gene Sequence, RFLP, Phylogenetic Tree
1. Introduction lentus isolated from healthy goat milk and dry-cured hams
were found to have the enterotoxigenic potential [11-14].
Members of Staphylococci are wide spread i
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