dispensabilities of carbonic anhydrase in proteobacteria可分配的碳酸酐酶在变形菌门.pdfVIP

Hindawi Publishing Corporation International Journal of Evolutionary Biology Volume 2012, Article ID 324549, 5 pages doi:10.1155/2012/324549 Research Article Dispensabilities of Carbonic Anhydrase in Proteobacteria Kenji Ueda,1 Hiromi Nishida,2 and Teruhiko Beppu1 1 Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-0880, Japan 2 Agricultural Bioinformatics Research Unit, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan Correspondence should be addressed to Kenji Ueda, ueda.kenji@nihon-u.ac.jp Received 10 February 2012; Accepted 14 March 2012 Academic Editor: Hideaki Nojiri Copyright © 2012 Kenji Ueda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Carbonic anhydrase (CA) (E.C. ) is a ubiquitous enzyme catalysing interconversion between CO2 and bicarbonate. The irregular distribution of the phylogenetically distinct classes of CA in procaryotic genome suggests its complex evolutionary history in procaryotes. Genetic evidence regarding the dispensability of CA under high-CO2 air in some model organisms indicates that CA-deficient microorganisms can persist in the natural environment by choosing high-CO2 niches. In this study, we studied the distribution of CA in the genome of Proteobacteria. While a large majority of the genome-sequenced Proteobacteria retained a CA gene(s), intracellular bacterial genera such as Buchnera and Rickettsia contained CA-defective strains. Comparison between CA-retaining and CA- deficient genomes showed the absence of whole coding seq