原创力文档differential regulation of the release of tumor necrosis factor-α and of eicosanoids by mast cells in rat airways after antigen challenge微分调节类花生酸的释放肿瘤坏死factor-α和肥大细胞在大鼠气道抗原的挑战.pdfVIP
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differential regulation of the release of tumor necrosis factor-α and of eicosanoids by mast cells in rat airways after antigen challenge微分调节类花生酸的释放肿瘤坏死factor-α和肥大细胞在大鼠气道抗原的挑战
Research Communication
Mediators of Inflammation, 12(4), 237/246 (August 2003)
BACKGROUND: Rat trachea display a differential topo- Differential regulation of the
graphical distribution of connective tissue mast cells
(CTMC) and mucosal mast cells (MMC) that may release of tumor necrosis factor-a
imply regional differences in the release of allergic and of eicosanoids by mast cells
mediators such as tumor necrosis factor-a (TNF-a)
and eicosanoids. in rat airways after antigen
Aim: To evaluate the role of CTMC and MMC for
release of TNF-a and eicosanoids after allergenic challenge
challenge in distinct segments of rat trachea.
Materials and methods: Proximal trachea (PT) and
distal trachea (DT) from ovalbumin (OVA)-sensitized 1 2
Aryene Goes Trezena , Zilma Lucia da Silva ,
rats, treated or not with compound 48/80 (48/80) or 2
Ricardo Martins Oliveira-Filho , Amilcar Sabino
dexamethasone, were incubated in culture medium.
Damazo3 4
After OVA challenge, aliquots were collected to study , Anita Hilda Straus , Helio Kiyoshi
release of TNF-a and eicosanoids. Takahashi4, Sonia Maria Oliani3 and Wothan Tavares
Results: Release of TNF-a by PT upon OVA challenge de Lima2,CA
peaked at 90 min and decayed at 6 and 24 h. Release
from DT peaked at 30/90 min and decayed 6 and 24 h
later. When CTMC were depleted with
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