development of time-resolved immunofluorometric assays for the detection of house dust mite-allergic ige in human sera发展的时间分辨immunofluorometric化验检测室内尘土mite-allergic ige人类血清.pdfVIP

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development of time-resolved immunofluorometric assays for the detection of house dust mite-allergic ige in human sera发展的时间分辨immunofluorometric化验检测室内尘土mite-allergic ige人类血清.pdf

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development of time-resolved immunofluorometric assays for the detection of house dust mite-allergic ige in human sera发展的时间分辨immunofluorometric化验检测室内尘土mite-allergic ige人类血清

Vol.2, No.11, 1280-1286 (2010) Health doi:10.4236/health.2010.211190 Development of time-resolved immunofluorometric assays for the detection of house dust mite-allergic IgE in human sera 1 1 2 Ratchanoo Phiphatchaipaisarn , Jundee Rabablert *, Kornkarn Bramarapravati , Duangthep Thongdee1 3 3 4 , Nares Wongpitoon , Worawan Durongpisitkul , Nat Malainual 1Department of Biology, Faculty of Science, Silpakorn University, Nakorn Pathom, Thailand; *Corresponding Author: jundee@su.ac.th ; 2Department of Preclinical Science, Faculty of Medicine, Thammasat University, Patumthani, Thailand; 3Department of Pediatric, Police General Hospital, Bangkok, Thailand; 4Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Received 6 September 2010; revised 15 September 2010; accepted 16 September 2010. ABSTRACT different research purposes. Dermatophagoides farinae and D. pteronyssi- Keywords: House Dust Mites; Time-Resolved nus are the prevalent house dust mites (HDM) in Immunofluorometric Assay; Allergy; IgE tropical countries and are associated with aller- gic diseases. This investigation developed a time- 1. INTRODUCTION resolved immunofluorometric assay (TR-IFMA) for the first time to detect specific IgE antibody House-dust mites (HDMs) represent one of the most in patients with skin prick te