dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-κb activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line英文论文.pdfVIP

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dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-κb activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line英文论文.pdf

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dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-κb activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line英文论文

Research Communication Mediators of Inflammation, 12(2), 71/78 (April 2003) AIMS : Nitric oxide (NO) has been increasingly im- Dexamethasone prevents plicated in inflammatory skin diseases, namely in allergic contact dermatitis. In this work, we investi- granulocyte/ macrophage colony- gated the effect of dexamethasone on NO production stimulating factor-induced nuclear induced by the epidermal cytokine granulocyte/ macrophage colony-stimulating factor (GM-CSF) in a factor-kB activation, inducible mouse fetal skin dendritic cell line.Methods: NO production was assessed by the method of Griess. nitric oxide synthase expression Expression of the inducible isoform of nitric oxide and nitric oxide production in a synthase (iNOS) protein was evaluated by western blot analysis and immunofluorescence microscopy. skin dendritic cell line Western blot analysis was also performed to evaluate cytosolic IkappaB-alpha (IkB-a) protein levels. The electrophoretic mobility shift assay was used to 1,2 CA,3 Ana Luısa Vital , Margarida Goncalo , ´ evaluate the activation or inhibition of nuclear factor ¸ M. Teresa Cruz1,2 3 kappa B (NF-kB).Results : GM-CSF induced iNOS ex- , Ame´rico Figueiredo , pression and NO production, and activated the Carlos B. Duarte2 and M. Celeste Lopes1,2 transcription factor NF-kB. Dexamethasone