developmental considerations of sperm protein 17 gene expression in rheumatoid arthritis synoviocytes发展考虑的精子蛋白17类风湿性关节炎synoviocytes基因表达.pdfVIP

Developmental Immunology, 2002 Vol. 9 (2), pp. 97–102 Developmental Considerations of Sperm Protein 17 Gene Expression in Rheumatoid Arthritis Synoviocytes a,b b a a b YUICHI TAKEOKA , THOMAS P. KENNY , HISASHI YAGO , MITSURU NAIKI , M. ERIC GERSHWIN and DICK L. ROBBINSb, * aInstitute of Bio-Active Science, Nippon Zoki Pharmaceuticals, Ltd., Hyogo, Japan; bDivision of Rheumatology/Allergy and Clinical Immunology, Department of Internal Medicine, University of California at Davis, Davis, CA 95616, USA Rheumatoid arthritis (RA) is an autoimmune disease characterized by proliferative synovial tissue. We used mRNA differential display and library subtraction to compare mRNA expression in RA and osteoarthritis (OA) synoviocytes. We initially compared the mRNA expression patterns in 1 female RA and 1 OA synovia and found a differentially expressed 350 bp transcript in the RA synoviocytes which was, by sequence analysis, 100% homologous to sperm protein 17 (Sp17). Moreover, the Sp17 transcript was found differentially expressed in a RA synovial library that was subtracted with an OA synovial library. Using specific primers for full length Sp17, a 1.1 kb transcript was amplified from the synoviocytes of 7 additional female RA patients, sequenced and found to 100% homologous to Sp17. Thus, we found the unexpected expression of Sp17, a thought to be gamete-specific protein, in the synoviocytes of 8/8 female RA patients in contrast to control OA synoviocytes. Interestingly, Sp17’s