development and validation of quechers method for estimation of propamocarb residues in tomato (lycopersicon esculentum mill) and soilquechers方法的开发和验证估计propamocarb残留茄(lycopersicon esculentum机)和土壤.pdfVIP

American Journal of Analytical Chemistry , 2011, 2, 26-31 doi:10.4236/ajac.2011.228120 Published Online December 2011 (http://www.SciRP.org/journal/ajac) Development and Validation of QuEChERS Method for Estimation of Propamocarb Residues in Tomato (Lycopersicon esculentum Mill) and Soil * Sanjay Kumar Sahoo , Raminderjit Singh Battu, Balwinder Singh Pesticide Residue Analysis Laboratory, Department of Entomology, Punjab Agricultural University, Ludhiana, India * E-mail : sksahoo_2006@ Received November 1, 2011; revised December 5, 2011; accepted December 16, 2011 Abstract An easy, simple and efficient analytical method was standardized and validated for the estimation of residues of propamocarb in tomato and soil. QuEChERS method included extraction of the sample with ethyl acetate and cleanup by treatment with PSA and graphatised carbon. Final clear extracts of ethyl acetate were con- centrated under vacuum to almost dryness and reconstituted into hexane. The residues of propamocarbwere estimated using gas chromatograph-mass spectrometry (GC-MS). Propamocarb presented a distinct peak at retention time of 8.962 min. Consistent recoveries of propamocarb ranging from 87 to 92 percent were ob- served when samples were spiked at 0.10, 0.50 and 1.00 mg·kg–1 levels. The limit of quantification (LOQ) of this method was determined to be 0.10 mg·kg–1 . Keywords: QuEChERS, Propamocarb, Tomato, Soil, GC-MS 1. Introduction curate and direct determination of propamocarb in com- mercial pesticides. The method can be used in the quan- P