development and validation of hplc method for simultaneous determination of amlodipine, valsartan, hydrochlorothiazide in dosage form and spiked human plasma开发和验证的高效液相色谱法同时测定氨氯地平缬沙坦氢氯噻嗪,剂型和人类血浆飙升.pdfVIP

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development and validation of hplc method for simultaneous determination of amlodipine, valsartan, hydrochlorothiazide in dosage form and spiked human plasma开发和验证的高效液相色谱法同时测定氨氯地平缬沙坦氢氯噻嗪,剂型和人类血浆飙升.pdf

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development and validation of hplc method for simultaneous determination of amlodipine, valsartan, hydrochlorothiazide in dosage form and spiked human plasma开发和验证的高效液相色谱法同时测定氨氯地平缬沙坦氢氯噻嗪,剂型和人类血浆飙升

American Journal of Analytical Chemistry , 2012, 3, 422-430 /10.4236/ajac.2012.36055 Published Online June 2012 (http://www.SciRP.org/journal/ajac) Development and Validation of HPLC Method for Simultaneous Determination of Amlodipine, Valsartan, Hydrochlorothiazide in Dosage Form and Spiked Human Plasma 1 2 3 3 Samya M. El-Gizawy , Osama H. Abdelmageed , Mahmoud A. Omar , Sayed M. Deryea , Ahmed M. Abdel-Megied4 1Analytical Chemistry Department, Faculty of Pharmacy, Assuit University, Assuit, Egypt 2Pharmaceutical Chemistry Department, Faculty of Pharmacy, King Abdulaziz University, Jeddah, KSA 3Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt 4Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 6th October City, Egypt Email: dr_ahmed80@ Received March 13, 2012; revised April 17, 2012; accepted April 27, 2012 ABSTRACT A simple, sensitive, and specific method was developed for simultaneous determination of Amlodipine besylate (AML), Valsartan (Vals) and Hydrochlorothiazide (HCT) by high performance liquid chromatography without previous separation. Satisfactory resolution was achieved using a RP-C18 chromatographic column, Phenomenex Kinetex (150 mm × 4.6 mm i.d) and a mobile phase consisting of acetonitrile-phosphate buffer (0.05 M) with pH 2.8 in the propor- tion of (40/60, v/v) at a flow rate 0.8 mL/min and the wavelength detection was 227 nm. The retention time for HCT,