dexamethasone-induced and estradiol-induced creb activation and annexin 1 expression in ccrf-cem lymphoblastic cells evidence for the involvement of camp and p38 mapk英文论文.pdfVIP

Research Communication Mediators of Inflammation, 12(6), 329/337 (December 2003) Aims: Annexin 1 (ANXA1), a member of the annexin Dexamethasone-induced and family of calcium-binding and phospholipid-binding proteins, is a key mediator of the anti-inflammatory estradiol-induced CREB activation actions of steroid hormones. We have previously and annexin 1 expression in CCRF- demonstrated that, in the human lymphoblastic CCRF-CEM cell line, both the synthetic glucocorticoid CEM lymphoblastic cells: evidence hormone, dexamethasone (Dex), and the estrogen for the involvement of cAMP and hormone, 17b-estradiol (E2b), induce the synthesis of ANXA1, by a mechanism independent of the activa- p38 MAPK tion of their nuclear receptors. Recently, it was reported that the gene coding for ANXA1 contains a cAMP-responsive element (CRE). In this work, we investigated whether Dex and E2b were able to induce M. Castro-Caldas1,CA, A. F. Mendes1,2, C. B. Duarte1 the activation of CRE binding proteins (CREB) in the and M. C. F. Lopes1,2 CCRF-CEM cells. Moreover, we studied the intracellu- lar signalling pathways involved in CREB activation and ANXA1 synthesis in response to Dex and E2b; 1 namely, the role of cAMP and the p38 mitogen- Centre for Neuroscience and Cell Biology, University activated protein kinase (MAPK). of Coimbra, 3004-517 Coimbra, Portugal and 2Faculty Results: The results show that Dex and E2b were as of Pharmacy, University of Coimbra, Portugal effective as the cAMP analogue, dBcAMP, in inducing CREB activation. On the contravy, dBcAMP induced ANXA1 synthesis as effectively as these steroid CA Corresponding author hormones. Furthermore