development and quality of bovine embryos produced in vitro using growth factor supplemented serum-free system开发和质量生产的牛胚胎体外无血清培养系统使用生长因子补充系统.pdfVIP

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development and quality of bovine embryos produced in vitro using growth factor supplemented serum-free system开发和质量生产的牛胚胎体外无血清培养系统使用生长因子补充系统.pdf

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development and quality of bovine embryos produced in vitro using growth factor supplemented serum-free system开发和质量生产的牛胚胎体外无血清培养系统使用生长因子补充系统

Vol.1, No.3, 97-105 (2011) Open Journal of Animal Sciences doi:10.4236/ojas.2011.13013 Development and quality of bovine embryos produced in vitro using growth factor supplemented serum-free system Arindam Dhali, Vahida M. Anchamparuthy, Steve P. Butler, Isis K. Mullarky, Ronald E. Pear- son, Frank C. Gwazdauskas* Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, USA; * Corresponding Author: guaz@ Received 1 July 2011; revised 10 August 2011; accepted 31 August 2011. ABSTRACT BCL-2, BAX, HSP1A1, GJA1 and BIRC5 tran- scripts varied significantly (P 0.05) in all cases, The influence of a growth factor supplemented except for BIRC5 in the blastocysts derived serum-free system on the development, gene from 3 - 10 mm follicles. Following thaw and expression, and cryotolerance of in vitro pro- culture, the development (%) of vitrified morulae duced bovine embryos was investigated. To into expanded/hatched blastocysts was lower (P assess the embryo development and gene ex- 0.01) with the supplementation of growth fac- pression in blastocysts, abattoir-derived oo- tors compared to FBS. In contrast, total number cytes (obtained from 3 - 10 or 3 mm follicles)