原创力文档distribution of cry and cyt genes among indigenous bacillus thuringiensis isolates with mosquitocidal activity哭,cyt分布与mosquitocidal在本土苏云金杆菌分离株的基因活动.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
distribution of cry and cyt genes among indigenous bacillus thuringiensis isolates with mosquitocidal activity哭,cyt分布与mosquitocidal在本土苏云金杆菌分离株的基因活动
Advances in Microbiology, 2012, 2, 216-226
/10.4236/aim.2012.23026 Published Online September 2012 (http://www.SciRP.org/journal/aim)
Distribution of cry and cyt Genes among Indigenous
Bacillus thuringiensis Isolates with Mosquitocidal Activity
1 1 2 2*
Ayyasamy Mahalakshmi , Kabilan Sujatha , Poornima Kani , Rajaiah Shenbagarathai
1UGC-NRCBS, School of Biological Sciences, Madurai Kamaraj University, Madurai, India
2PG and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai, India
*
Email: shenbagarathai@
Received June 11, 2012; revised July 5, 2012; accepted July 15, 2012
ABSTRACT
Bacillus thuringiensis strains isolated from Madurai, TamilNadu, India were evaluated for their mosquitocidal activity,
as well as cry and cyt genes diversity. It revealed that 99% of the parasporal crystal morphology of these isolates was
spherical in nature and a variable percentage (0% - 100%) of toxicity was observed against Culex quinquefasciatus and
Aedes aegypti. PCR analysis revealed that 53% of the isolates were positive for the various cry and cyt genes tested,
whereas 47% did not produce any PCR product for the cry gene analyzed. Diverse pattern of cry and cyt genes distribu-
tion was observed even in the isolates from the same sample. B. thuringienis subsp. LDC-9 showed three-fold higher
toxicity against Culex quinquefasciatus than that of B. thuringiensis var israelensis which might be used as a potential
strain to control mosquitoes in near future after field evaluation.
Keywords: Bacillus thuringiensis ; Crystal Endotoxin; Culex quinquefasciatus; Aedes aegypti
1. Introduction
您可能关注的文档
- direct determination of radionuclides in the body optimisation of measurements parameters and results analysis直接测定体内放射性核素的测量参数的优化和结果分析.pdf
- direct computation of operational matrices for polynomial bases直接计算操作矩阵多项式基地.pdf
- direct detection of thrombin binding to 8-bromodeoxyguanosine-modified aptamer effects of modification on affinity and kinetics直接检测凝血酶结合8-bromodeoxyguanosine-modified适配子修改亲和力和动力学的影响.pdf
- direct mechanisms, menus and latent contracts直接的机制,菜单和潜在的合同.pdf
- direct growth of high-quality inp layers on gaas substrates by mocvd在砷化镓基板上直接生长高质量的输入层由金属.pdf
- direct and inverse approximation theorems for baskakov operators with the jacobi-type weight直接和逆近似定理baskakov运营商jacobi-type重量.pdf
- direct fisher inference of the quartic oscillator’s eigenvalues四次振荡器直接费舍尔推理的特征值.pdf
- direct decomposition of no into n2 and o2 over c-type cubic y2o3-tb4o7-zro2没有直接分解成n2和o2 c -型立方y2o3-tb4o7-zro2.pdf
- direction finding using cumulant in multipath environment with an arbitrary array测向在多径环境中使用累积量与任意数组.pdf
- direct optical energy gap in amorphous silicon quantum dots直接在非晶硅量子点光学能隙.pdf
- distribution of maps with transversal homoclinic orbits in a continuous map space与同宿轨横向分布地图连续映射空间.pdf
- distribution of 5-hydroxymethylcytosine in different human tissues5-hydroxymethylcytosine分布在不同的人体组织.pdf
- distribution of special sequences modulo a large prime分布的特殊序列模一个大素数.pdf
- distributions of pigments in reef sediments, contribution of phytoplankton to organic matter budget in coral reef分布在礁沉积色素,浮游植物的贡献在珊瑚礁有机质预算.pdf
- distribution of geometrically weighted sum of bernoulli random variables几何分布的加权和伯努利随机变量.pdf
- distribution of roots of polynomial congruences多项式的根的分布刻画.pdf
- distributions of ratios from random variables to random matrices从随机变量分布的比例随机矩阵.pdf
- distributional similarity for chinese exploiting characters and radicals中国利用字符和激进分子的分布相似.pdf
- divalent cu, cd, and pb biosorption in mixed solvents二价铜、cd和pb生物吸附在混合溶剂.pdf
- distributions of radionuclides (u & th) and pedogenic characteristics as indicators of wet and warm climate during the holocene in the western part of the upper gangetic plain, india放射性核素的分布(u & th)和成土的特征指标的潮湿和温暖的气候在西部全新世期间恒河平原上,印度.pdf
文档评论（0）