胰泰复方对慢性胰腺炎所致纤维化大鼠血清MDA含量血小板源性生长因子PDGF-B表达影响.docVIP

胰泰复方对慢性胰腺炎所致纤维化大鼠血清MDA含量血小板源性生长因子PDGF-B表达影响(1.北京中医药大学2008级博士研究生,北京 100029;2.黑龙江中医药大学附属医院,黑龙江 哈尔滨 150040; 3.中日友好医院国家中医药管理局重点研究室,北京 100029;4.黑龙江中医药大学基础医学院,黑龙江 哈尔滨 150040) 摘 要:目的:观察中药胰泰复方对慢性胰腺炎胰腺纤维化大鼠血清丙二醛(MDA)和血小板源性生长因子B(PDGF-B)表达的影响。方法:以L-精氨酸左下腹腔注射制备大鼠慢性胰腺炎模型,予相应药物灌胃治疗,2个月后取血清用生化检测法测定MDA含量,取胰腺组织用免疫组织化学法测定PDGF-B的含量。结果:模型组MDA、PDGF-B含量明显高于空白组、中药对照组及中药治疗组,中药治疗组MDA、PDGF-B含量低于中药对照组。结论:胰泰复方能降低胰腺组织中MDA和PDGF-B的含量,对慢性胰腺炎胰腺纤维化具有恢复作用。 关键词:胰泰复方;慢性胰腺炎;胰腺纤维化;血清丙二醛(MDA);血小板源性生长因子B(PDGF-B)? 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)04-0825-03 Effect of YITAI Compounded Medication on Expression of Maleic Dialdehyde And Platelet Derived Growth Factor-B of the Rats with Chronic Pancreatitis Fibrosis CHEN Xin?xin 1, ZHOU Jing?hua2,LI You?lin3,LIU Hua?sheng4 (1.Medical Doctoral Student of 2008 of Beijing University of Chinese Medicine,Beijing 100029,China; 2.Pathologicai Ward of Hospital Affiliated to Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,Heilongjiang,China; 3.Focus Investigatory office of China-Japan Friendship Hospital and State Administration of Traditional Chinese Medicine of the People’s Republic of China,Beijing 100029,China; 4.Basic medical College of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,Heilongjiang,China.) Abstract:?Objective?:To investigate the influences of YITAI compounded medication on the expression of maleic dialdehyde and platelet derived growth factor-B of the model rats with chronic pancreatitis fibrosis.?Methods?:To establish the chronic pancreatitis rats model with L-arginine with the method of celia injection, we gave them YITAI compounded medication and collected serum and pancreas to check the contents of MDA and PDGF-B with the method of immune histochemistry.?Results?:The contents of MDA in serum and PDGF-B in model group was obviously higher than normal group,Chinese medicine control group and Chinese medicine treatment group,the contents of MDA in serum and