effect of k1, k2 anti-bacterial agents on tobacco ralstonia solanacearumk1、k2抗菌药物对烟草ralstonia solanacearum.pdfVIP

Engineering, 2010, 2, 930-934 doi:10.4236/eng.2010.211117 Published Online November 2010 (/journal/eng). Effect of K1, K2 Anti-Bacterial Agents on Tobacco Ralstonia Solanacearum 1 1 1 1* Anan Wang , Zhifeng Zhao , Zhenzhen Liu , Yixin Liu School of Earth and Space Science, University of Science and Technology of China, Hefei, China E-mail : wanganan@, liuyixin@ Received July 30, 2010; revised October 8, 2010; accepted October 26, 2010 Abstract The tobacco Ralstonia Solanacearum were both cultured on nutrient agar plates and inoculated in seedling stage of tobacco, then treated with K1 and K2, two anti-bacterial agents, at a serial concentrations to study their inhibitory efficiency. The result indicated that K1 can inhibit R. Solanacearum growth entirely, at the concentration range from 1/50 to 1/5000. K2 can reach the same result at the concentration range from 1/50 to 1/50000. Compared with the control plates, K1, at the concentration 1/50000, had no significant differ- ences, and the average number of colony per plate was 112-115. The immature tobacco shown wilt as soon as inoculated with R. Solanacearum, and recovered gradually after using K1, K2. The densities of microbial suspension, handled by K1, K2 within 10 hs, were both significantly lower than the controlled ones. The op- tical microscopy also shown that handled microbial body differed from the controlled, whose body was regular short, rod shape as opposed to the handled ones with irregular rod shape and damaged body. All the results indicated that K1 and K2 both had inhibitory effects on tobacco R. Solanacearum, and K2 was more efficient than K1. Keywords: Anti-Bacterial Agents K1, K2, Inhibition,