原创力文档effect of l-lysine in culture medium on nodule formation by bone marrow cells赖氨酸在培养基的影响由骨髓细胞结节形成.pdfVIP
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effect of l-lysine in culture medium on nodule formation by bone marrow cells赖氨酸在培养基的影响由骨髓细胞结节形成
J. Biomedical Science and Engineering, 2012, 5, 587-592 JBiSE
/ 10.4236/jbise.2012.510072 Published Online October 2012 (http://www.SciRP.org/journal/jbise/)
Effect of L-lysine in culture medium on nodule formation
by bone marrow cells
Masataka Yoshikawa, Yasunori Shimomura, Hideyuki Kakigi, Norimasa Tsuji, Takayoshi Yabuuchi,
Hiroyuki Hayashi
Department of Endodontics, Osaka Dental University, Osaka, Japan
Email: yosikawa@cc.osaka-dent.ac.jp, shimoyan3@, kakigi@cc.osaka-dent.ac.jp, n-tsuji@cc.osaka-dent.ac.jp,
takayan0813@yahoo.co.jp, hayashi@cc.osaka-dent.ac.jp
Received 25 July 2012; revised 26 August 2012; accepted 10 September 2012
ABSTRACT sary before the actual utilization of regenerated teeth
produced by tissue engineering. For tooth regeneration,
The aim of this study was to estimate the effect of stem cells as progenitor cells or odontoblasts are rquired.
L-lysine on nodule formation by rat bone marrow It is considered that the most desirable source of cells
cells in vitro. In this study, L-lysine was added to me- may be the dental pulp. However, there are not many
dium for mesenchymal stem cell culture to promote opportunities to extract teeth. We can only obtain dental
proliferation and differentiation of the cells, and then pulp from sound teeth which must be extracted owing to
nodule formation was estimated in an in vitro rat orthodontic requirements. Moreover, the isolation of
bone marrow cell culture. Bone marrow cells from odontoblasts from dental pulp may be too difficult.
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