effect of magnolol on the function of osteoblastic mc3t3-e1 cells自由基对成骨细胞的功能的影响mc3t3-e1细胞.pdfVIP

Hindawi Publishing Corporation Mediators of Inflammation Volume 2012, Article ID 829650, 7 pages doi:10.1155/2012/829650 Research Article Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells Eun Jung Kwak,1 Young Soon Lee,2 and Eun Mi Choi2 1 Department of Food Science Technology, Yeungnam University, Gyeongsan 712-749, Republic of Korea 2 Department of Food Nutrition, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea Correspondence should be addressed to Eun Mi Choi, cheunmi@ Received 24 August 2011; Accepted 6 November 2011 Academic Editor: Rwei-Fen S. Huang Copyright © 2012 Eun Jung Kwak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Objectives. In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia offi cinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells. Methods. Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor-κB ligand (RANKL), TNF-α, and IL-6 were measured with an enzyme immunoassay system. Results. Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells (P 0.05). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF-α, and IL-6 were found to be key osteoclas