effect of matrix clean-up for aflatoxin analysis in corn and dried distillers grains影响黄曲霉毒素分析矩阵清理的玉米和谷物蒸馏器干.pdfVIP

Natural Resources, 2011, 2, 250-257 doi:10.4236/nr.2011.24032 Published Online December 2011 (http://www.SciRP.org/journal/nr) Effect of Matrix Clean-Up for Aflatoxin Analysis in Corn and Dried Distillers Grains 1,2 3 4 2 1,2* 1,2* A. McDaniel , W. E. Holmes , P. Williams , K. L. Armbrust , D. L. Sparks , A. E. Brown 1Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Starkville, USA; 2Office of the State Chemist, Mississippi State University, Starkville, USA; 3 Swalm School of Chemical Engineering, Mississippi State University, Starkville, USA; 4USDA ARS Corn Host Plant Resistance Research Unit, Washington DC, USA. * Email: abrown@ Received September 24th, 2011; revised October 15th, 2011; accepted October 28th, 2011. ABSTRACT Aflatoxins are a group of highly carcinogenic mycotoxins that contaminate a wide variety of agricultural crops and have a detrimental economic impact on industries, such as corn and ethanol production. They are regulated by the FDA, and therefore, rapid, reliable cleanup techniques with low detection limits are needed for aflatoxins in a wide array of matri- ces. In this study the effect of using an immunoaffinity column versus simple filtering as a cleanup was tested for aflatox- ins extracted from corn and Dried Distillers Grains (DDG). The aflatoxins were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The use of an immunoaffinity column resulted in greater signal-to-noise ratios (S/N), S/N of 70 vs S/N of 5 for corn, as well as fewer non-target peaks in the analysis. Recoveries of aflatoxin using im- munoaffinity ranged from 40% to 104.5% (spiked substrate) and 49% to 120% (spiked extract) while percent recoveries of filtered samples ranged from 84