early appearance of tnf-α and other cytokines in bronchus associated lymphoid tissues (balt) from growing wistar rats. what is the role of tnf-α早期出现tnf-α和其他细胞因子在支气管相关淋巴组织(balt)从种植wistar鼠。.pdfVIP

Clinical Developmental Immunology, September/December 2004, Vol. 11 (3/4), pp. 253–259 Early Appearance of TNF-a and Other Cytokines in Bronchus Associated Lymphoid Tissues (BALT) from Growing Wistar Rats. What is the Role of TNF-a? DREANINA DELETTIERES, CECILIA A. FRECHA and MARIA E. ROUX* Faculty of Pharmacy and Biochemistry, Laboratory of Cellular Immunology, Department of Biological Sciences, University of Buenos Aires, Junin 956, 5th floor, C1113AAD Buenos Aires, Argentina Several different cytokines trigger the development of determined cell subsets in BALT of growing Wistar rats. Early appearance (4 days post partum) of gdT cells in BALT has been shown, as well as its role in up-regulating TNF-a production. In the present report, we studied in the BALT: (1) the profile of the cytokines, TNF-a, INF-g and IL-10 and (2) in TCR gdþ cells, the existence of a colocalization with TNF-a as well as with INF-g. All the cytokines studied were observed at an early stage of BALT development by immunohistochemistry and in bronchoalveolar cells (BAL cells) by flow cytometry and western blot. (1) The principal cytokine found at 4 days of age in BALT cells was TNF-a that increases along BALT development. The same behavior was found for cells containing IL-10 and INF-g. (2) TCR gdþ cells colocalize mainly with TNF-a as it has been shown by immunohistochemistry in BALT and by flow cytometry when we studied BAL. The early appearance of TNF-a concomitant with TCR gdþ cell suggests an important role for this cytokine along BALT development. Moreover, mutual regulation between them exists taking part in th