艾灸增强溃疡性结肠炎大鼠穴区HSP72及IL-6表达.docVIP

艾灸增强溃疡性结肠炎大鼠穴区HSP72及IL-6表达摘 要:目的:通过观察艾灸对溃疡性结肠炎(UC)模型大鼠穴区HSP72、IL-6表达,探讨艾灸后穴区HSP72和IL-6的表达是否有变化,如果艾灸后其表达有变化,这种变化与艾灸的刺激量之间是否相关。方法:采用免疫学方法加局部刺激制备实验性UC大鼠模型,确定造模成功后,随机分模型组,艾灸A组,艾灸B组,艾灸C组,每组8只,选取天枢穴施以不同灸量的麦粒灸治疗并设立正常对照组。治疗结束后,HE染色观察结肠病变区及免疫组化染色法观察天枢穴区HSP72和IL-6的表达。结果:(1)与正常组比较,模型组大鼠结肠病理变化明显;艾灸治疗后,大鼠结肠病理变化明显改善。(2)造模后,模型组穴区HSP72和IL-6表达降低,与正常组比较,模型组阳性目标总面积具有统计学意义(?P? 所有数据用均数±标准差(?±s?)表示,运用SPSS 10.0统计软件进行统计学处理。对数据作正态分布检验,符合正态性分布用Oneway Analysis Of Variance(One-way ANOVA)进行分析,不符合正态性分布用非参数检验。方差齐同时采用最小显著差法(Least significant difference, LSD)比较组间差异性,方差不齐时用Games-Howell比较组间差异性。?P? 溃疡性结肠炎主要表现为肠道炎症反应,与免疫功能紊乱关系密切。值得注意的是,与正常组比较,UC模型组HSP72和IL-6表达均降低,且阳性目标总面积均有统计学意义(?P? [17] Joos S, Wildau N, Kohnen R, et al. Acupuncture and moxibustion in the treatment of ulcerative colitis: a randomized controlled study [J]. Scand J Gastroenterol, 2006, 41(9):1056-1063. [18] Wu HG, Liu HR, Tan LY, et al. Electroacupuncture and moxibustion promote neutrophil apoptosis and improve ulcerative colitis in rats [J]. Dig Dis Sci, 2007, 52(2):379-384. [19] Wu HG, Zhou LB, Pan YY, et al. Study of the mechanisms of acupuncture and moxibustion treatment for ulcerative colitis rats in view of the gene expression of cytokines [J]. World J Gastroenterol, 1999, 5(6):515-517. [20] 徐叔云,卞如濂,陈修. 药理实验方法学[M].北京:人民卫生出版社,2001:1335. [21] Jonak C, Klosner G, Trautinger F. Heat shock proteins in the skin [J]. Int J Cosmet Sci, 2006, 28(4):233-241. [22] Gong B, Asimakis GK, Chen ZP, et al. Whole-body hyperthermia induces up-regulation of vascular endothelial growth factor accompanied by neovascularization in cardiac tissue [J]. Life Sciences, 2006, 79(19): 1781-1788. [23] Maytin EV. Differential effects of heat shock and UVB light upon stress protein expression in epidermal keratinocytes [J]. J Biol Chem, 1992, 267(32):23189-23196. [24] Trautinger F, Trautinger I, Kindas-Mügge I, et al. Human keratinocytes in vivo and in vitro constitutively express the 72-kD heat shock protein